Early establishment of hematopoietic chimerism following allogeneic peripheral blood stem cell transplantation in comparison with allogeneic bone marrow transplantation
Early establishment of hematopoietic chimerism following allogeneic peripheral blood stem cell transplantation in comparison with allogeneic bone marrow transplantation
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异体外周血干细胞移植与异体骨髓移植较早建立造血嵌合状态
DOI：
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发表时间：
1999
影响因子：
3.1
通讯作者：
M. Harada
中科院分区：
文献类型：
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作者：
S. Nakao;W. Zeng;H. Yamazaki;Honbo Wang;A. Takami;N. Sugimori;Y. Miura;S. Shiobara;T. Matsuda;Yuji Shinagawa;M. Harada
翻译后摘要：为了表征异基因外周血干细胞移植（allo-PBSCT）后建立完全嵌合体的过程中，我们确定了从23例患者的外周血（PB）中的白细胞的起源在allo-PBSCT后的非常早期使用基因组DNA的迷你或微卫星区域的扩增。 从19例allo-PBSCT患者移植后第8天获得的PB中扩增供体特异性等位基因。在19例患者中，12例仅显示供体特异性等位基因（完全嵌合体），7例同时显示供体和宿主特异性等位基因（混合嵌合体）。尽管12例接受异基因骨髓移植（allo-BMT）的患者中有10例与allo-PBSCT相似地扩增了供体特异性等位基因，但这10例患者均显示混合嵌合体。当在移植后2-3天间隔连续获得的PB样本中检查嵌合状态时，allo-PBSCT患者中的宿主特异性等位基因在PB中检测不到比allo-BMT患者早得多。这些结果表明，与allo-BMT相比，allo-PBSCT后供体来源细胞的出现与宿主来源细胞在循环中的消失相关，导致完全嵌合体的快速建立。
Abstract: To characterize the process of the establishment of complete chimerism after allogeneic peripheral blood stem cell transplantation (allo‐PBSCT), we determined the origin of leukocytes in peripheral blood (PB) obtained from 23 patients in the very early period after allo‐PBSCT using amplification of mini‐ or microsatellite regions of genomic DNA. Donor‐specific alleles were amplified from the PB obtained at day 8 post‐transplant for 19 allo‐PBSCT patients. Among the 19 patients, 12 showed only donor‐specific alleles (complete chimerism) while 7 did both donor and host‐specific alleles (mixed chimerism). Although donor specific alleles were amplified in 10 of 12 patients who received allogeneic bone marrow transplantation (allo‐BMT) similarly to allo‐PBSCT, all of these ten showed mixed chimerism. When the chimeric state was examined in PB samples obtained serially at 2–3‐day intervals post‐transplant, host‐specific alleles in allo‐PBSCT patients were not detectable in the PB much earlier than those in allo‐BMT patients. These findings indicate that the appearance of donor‐derived cells associated with the disappearance of host‐derived cells in the circulation occurs earlier after allo‐PBSCT as compared with allo‐BMT, leading to the rapid establishment of complete chimerism.
来自 G-CSF 处理的供体的同种异体外周血细胞实验性移植后的长期植入、移植物抗宿主病和免疫重建。
DOI：
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发表时间：
1996
期刊：
Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation.
影响因子：
--
作者：
Pan,L;Bressler,S;Cooke,KR;Krenger,W;Karandikar,M;Ferrara,JL
通讯作者：
Ferrara,JL
影响因子：
20.3
作者：
L. Ugozzoli;P. Yam;LD Petz;GB Ferrara;RE Champlin;SJ Forman;D. Koyal;RB Wallace
通讯作者：
L. Ugozzoli;P. Yam;LD Petz;GB Ferrara;RE Champlin;SJ Forman;D. Koyal;RB Wallace
影响因子：
20.3
作者：
Giralt, S;Estey, E;Champlin, R
通讯作者：
Champlin, R