Fungal symbiont community and absence of detectable mycangia in invasive Euplatypus ambrosia beetles
Fungal symbiont community and absence of detectable mycangia in invasive Euplatypus ambrosia beetles
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DOI：
10.1007/s13199-023-00938-4
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发表时间：
2023-09-18
期刊：
影响因子：
2.5
通讯作者：
Hulcr，Jiri
中科院分区：
文献类型：
--
作者：
Rodrigues，Andre;Johnson，Andrew J.;Hulcr，Jiri
桔梗甲虫以互惠共生的真菌为食物，双方合作定居枯树。真菌在特殊的结构(霉菌)中运输，但在许多鸭嘴兽物种中，霉菌的位置未知。一种难以捉摸的霉菌是平腹真甲藻，广泛分布于美洲，最近在全世界范围内入侵。根据对其他毒蛾的了解，我们预测霉囊可能在头部内部、前胸内或外，或者共生体在后肠内。我们尝试使用X射线计算机体层摄影术、荧光原位杂交和组织学进行检测。为了进行方法验证和比较，我们使用了与前鼻部霉菌病有亲缘关系的合成真甲藻。尽管对两性的文昌菌进行了常规分离，但在E内的任何地方都没有发现一致的菌囊状结构。平行线。这两种真甲虫物种在不同的身体部位都产生了不同的真菌群落，但这两种甲虫物种中最一致的，也是最有可能的营养互惠物种是Raffaelea xyleborini。一个值得注意的发现是，在这两个物种的扩散过程中，雌性动物的后肠充满了大量紧密堆积的酵母菌，其中大部分是未知的Starmeras种。这种酵母缓存的功能尚不清楚。我们的结果表明，这两种真平菇都与同一种真菌BUT有关。Parallus要么没有霉囊，要么我们找不到它们。这项研究增加了越来越多的证据，表明扁形甲虫已经与Raffaelea属的成员共同进化，并且它们在属的水平上是混杂的。
Platypodinae ambrosia beetles depend on mutualistic fungi for food, and both partners cooperate in colonizing dead trees. The fungi are transported in specialized structures (mycangia), but the location of mycangia is unknown in many platypodine species. One species with elusive mycangia isEuplatypus parallelus, widespread in the Americas, and recently invasive worldwide. Drawing on knowledge about other ambrosia beetles, we predict that the mycangia may be either internal in the head, internal or external within the prothorax, or the symbiont is carried within the hindgut. We attempted detection using X-ray computed tomography, Fluorescence In Situ Hybridization and histology. For method validation and comparison we usedEuplatypus compositus, a related species with pronotal mycangia. Despite routine isolation of the ambrosia fungi from both sexes, no consistent mycangia-like structures were found anywhere withinE. parallelus. BothEuplatypusspecies yielded a diverse fungal community on different body parts, but the most consistent associate of both beetle species, and the most likely nutritional mutualist, isRaffaelea xyleborini. A notable discovery is that during dispersal in both species, females had their hindgut filled with a mass of tightly packed yeasts, mostly an unknownStarmeraspecies. The function of this yeast cache is not known. Our results showed that bothEuplatypusspecies are associated with the same fungus, butE. paralleluseither does not have mycangia or we failed to locate them. This study adds to the growing evidence that Platypodinae beetles have coevolved with members of the genusRaffaeleaand that they are promiscuous at the genus level.