Expression analysis of global gene response to chronic heat exposure in broiler chickens (Gallus gallus) reveals new reactive genes
Expression analysis of global gene response to chronic heat exposure in broiler chickens (Gallus gallus) reveals new reactive genes
复制标题
DOI：
10.3382/ps.2010-01144
复制
发表时间：
2011-05-01
期刊：
影响因子：
4.4
通讯作者：
Wu, C.
中科院分区：
文献类型：
--
作者：
Li, C.;Wang, X.;Wu, C.
热调节的过程是复杂的，确切的分子机制尚未完全了解。为研究慢性热暴露对基因表达的影响，构建了丝羽乌骨鸡胸肌和肝脏组织cDNA文库，并进行了生物信息学分析。共鉴定出8，935个非冗余EST，并用于基因表达分析。基因芯片分析表明，在肉鸡（Gallus gallus）胸肌中，110个基因的表达水平变化后，3周的循环热应激。泛素B（UBB）;泛素C（UBC）;肿瘤坏死因子受体相关因子3相互作用Jun氨基末端激酶激活调节剂TRAF 3 IP 3、EIF 3S 6、PABPC 1和FBXO 11是已知参与热调节的唯一基因;大多数其他基因首次被证明是相关的。新的热反应性基因的发现，使人的细胞分裂原活化蛋白激酶激活蛋白PM20/PM21、细胞因子信号抑制因子（SOCS）盒蛋白2（ASB 2）、泛素特异性蛋白酶45（USP 45）、和TRK融合基因（TFG）的垂直关系表明，丝裂原活化蛋白激酶途径以及泛素-蛋白酶体途径和核因子κ B途径在热调节中起重要作用。这项研究为热应激的调控提供了新的信息，尽管其机制还远未被理解。需要对新发现的热反应基因进行进一步深入研究，以充分了解其在体温调节中的分子功能。
The process of heat regulation is complex and the exact molecular mechanism is not fully understood. To investigate the global gene response to chronic heat exposure, a breast muscle cDNA library and a liver tissue cDNA library from Silkie fowl were constructed and analyzed in bioinformatics. A total of 8,935 nonredundant EST were identified from and used for gene expression analysis. Microarray assay revealed that in breast muscle of broiler chickens (Gallus gallus), 110 genes changed expression levels after 3 wk of cycling heat stress. Ubiquitin B (UBB); ubiquitin C (UBC); tumor necrosis factor receptor-associated factor 3-interacting Jun amino-terminal kinase activating modulator (TRAF3IP3); eukaryotic translation initiation factor 3, subunit 6 (EIF3S6); poly(A) binding protein, cytoplasmic 1 (PABPC1); and F-box only protein 11 (FBXO11) were the only genes that have been reported to be involved in heat regulation; the majority of the other genes were shown to be related for the first time. The finding of new heat-reactive genes left perpendicularmitogen-activated protein kinase activating protein PM20/PM21; suppressors of cytokine signaling (SOCS) box-containing protein 2 (ASB2); ubiquitin-specific proteinase 45 (USP45); and TRK-fused gene (TFG)right perpendicular suggests that the mitogen-activated protein kinase pathways as well as the ubiquitin-proteasome pathways and the nuclear factor kappa B pathways play important roles in heat regulation. This study provides new information on the regulation of heat stress, though the mechanism is far from being understood. Further in-depth research on the newly discovered heat-reactive genes is required to fully understand their molecular functions in thermoregulation.