Cultured mucosal cell sheet with a double layer of keratinocytes and fibroblasts on a collagen membrane
Cultured mucosal cell sheet with a double layer of keratinocytes and fibroblasts on a collagen membrane
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DOI：
10.1089/1076327041348329
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发表时间：
2004-05-01
影响因子：
--
通讯作者：
Omura, K
中科院分区：
文献类型：
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作者：
Imaizumi, F;Asahina, I;Omura, K
本研究的目的是开发一种易于制备和操作的新型培养粘膜，可应用于口腔粘膜缺损。从口腔粘膜制备人口腔角质形成细胞和成纤维细胞。我们制作了以下两种类型的培养粘膜细胞片：在胶原膜上培养的单层角质形成细胞片（K-S）和在胶原膜上培养的角质形成细胞和成纤维细胞的双层片（KF-S）。使用胶原膜作为对照。将每种类型的片材移植到裸鼠的背部皮肤缺损处。测量伤口面积以评估伤口收缩，并在移植后1周和4周采集样本以进行组织学评估。 KF-S 移植物的伤口收缩最小。尽管组织学检查显示所有移植类型的上皮分化正常，但 KFS 的外皮蛋白表达模式与正常上皮最相似。这些结果表明，在胶原膜上培养的双层角质形成细胞和成纤维细胞可以促进上皮愈合并防止伤口收缩。
The aim of this study was to develop a novel cultured mucosal membrane that was facile to prepare and easy to handle, and that could be applied to mucosal defects in the oral cavity. Human oral keratinocytes and fibroblasts were prepared from the oral mucosa. We made the following two types of cultured mucosal cell sheets: a monolayer sheet of keratinocytes cultured on a collagen membrane (K-S) and a double-layered sheet of keratinocytes and fibroblasts on a collagen membrane (KF-S). A collagen membrane was used as a control. Each type of sheet was transplanted onto dorsal skin defects of nude mice. The wound area was measured for the assessment of wound contraction and a specimen was harvested for histologic evaluation 1 week and 4 weeks after grafting. Wound contraction was minimal with KF-S grafts. Although histologic examination showed normal differentiation of the epithelium in all graft types, the involucrin expression pattern of KFS was most similar to that of normal epithelium. These results indicate that a double-layered sheet of keratinocytes and fibroblasts cultured on a collagen membrane may facilitate epithelial healing and prevent wound contraction.