Mechanisms of antibiotic resistance in Escherichia coli isolates recovered from wild animals
Mechanisms of antibiotic resistance in Escherichia coli isolates recovered from wild animals
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DOI：
10.1089/mdr.2008.0795
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发表时间：
2008-03-01
影响因子：
2.6
通讯作者：
Torres, Carmen
中科院分区：
文献类型：
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作者：
Costa, Daniela;Poeta, Patricia;Torres, Carmen
在Levine琼脂平板（未添加抗生素）上采集72份来自葡萄牙野生动物的粪便样本，从其中56份（78%）中回收大肠杆菌分离株，共获得112株大肠杆菌。大肠杆菌分离株（每个样品两个）。这些菌株对16种抗生素的敏感性进行了研究，获得了以下耐药百分比：四环素，链霉素，氨苄青霉素和甲氧苄啶-磺胺甲恶唑（SXT）（范围19-35%）;萘啶酸（14%）;环丙沙星（9%）;阿莫西林-克拉维酸、庆大霉素、妥布霉素和氯霉素（范围4.5-7%）;头孢噻肟和氨曲南（1.8%）;头孢他啶（0.9%）;阿米卡星、头孢西丁和亚胺培南（0%）。25株氨苄西林耐药菌株中有22株携带bla（TEM）基因，其中2株携带CTX-M-14 β-内酰胺酶基因，其中1株携带bla（TEM-52）基因。检测到的其他耐药基因如下：所有庆大霉素耐药分离株中存在aac（3）-II或aac（3）-IV基因; 25株链霉素耐药分离株中有22株存在aadA 1或aadA 2;所有39株四环素耐药分离株中存在泰特（A）和/或泰特（B）;所有21株SXT耐药分离株中存在sul 1和/或sul 2和/或sul 3基因。GyrA蛋白的两个氨基酸变化（Ser 83 Leu + Asp 87 Asn）和ParC蛋白的一个变化（Ser 80 Ile）在我们的系列的所有10个环丙沙星耐药菌株中被鉴定。野生动物的肠道是抗生素耐药基因的储存库，尤其是对氨苄青霉素、四环素、链霉素和SXT的耐药基因，多重耐药大肠杆菌的存在也是值得注意的。在一些受试动物中检测到大肠杆菌分离株。
Seventy-two fecal samples obtained from wild animals in Portugal were sampled on Levine agar plates (non-supplemented with antibiotics), and Escherichia coli isolates were recovered from 56 of them (78%), obtaining a total of 112 E. coli isolates (two per sample). Susceptibility to 16 antibiotics was studied in these isolates, and the following percentages of resistance were obtained: tetracycline, streptomycin, ampicillin, and trimethoprim-sulfamethoxazole (SXT) (range 19-35%); nalidixic acid (14%); ciprofloxacin (9%); amoxicillin-clavulanic acid, gentamicin, tobramycin, and chloramphenicol (range 4.5-7%); cefotaxime, and aztreonam (1.8%); ceftazidime (0.9%); and amikacin, cefoxitin, and imipenem (0%). A bla(TEM) gene was found in 22 of the 25 ampicillin-resistant isolates, and the gene encoding CTX-M-14 beta-lactamase was identified in the two cefotaxime-resistant isolates (recovered from a common kestrel and a sparrowhawk), associated with bla(TEM-52) gene in one of them. Other resistance genes detected were as follows: aac(3)-II or aac(3)-IV genes in all gentamicin-resistant isolates; aadA1 or aadA2 in 22 of 25 streptomycin-resistant isolates; tet(A) and/or tet(B) in all 39 tetracycline-resistant isolates; and sul1 and/or sul2 and/or sul3 genes in all 21 SXT-resistant isolates. Two amino acid changes in GyrA protein (Ser83Leu + Asp87Asn) and one change in ParC protein (Ser80Ile) were identified in all 10 ciprofloxacin-resistant isolates of our series. The intestinal tract of wild animals is a reservoir of antibiotic resistance genes, especially for ampicillin, tetracycline, streptomycin, and SXT, and it is also remarkable that multiresistant E. coli isolates are detected in some of the tested animals.