Quorum sensing inhibition as a promising method to control biofilm growth in metalworking fluids
Quorum sensing inhibition as a promising method to control biofilm growth in metalworking fluids
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DOI：
10.1007/s10295-019-02181-7
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发表时间：
2019-04
影响因子：
3.4
通讯作者：
Safiye Selen Özcan;Markus Dieser;A. Parker;Narayanaganesh Balasubramanian;C. Foreman
中科院分区：
文献类型：
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作者：
Safiye Selen Özcan;Markus Dieser;A. Parker;Narayanaganesh Balasubramanian;C. Foreman
金属加工系统中的微生物污染是一个关键问题。本研究确定了两个加工车间的金属加工液 (MWF) 中的微生物群落，并研究了群体感应抑制 (QSI) 对生物膜生长的影响。在这两项操作中，生物膜相关和浮游微生物群落以假单胞菌为主（60.2-99.7%）。即使在倾倒用过的金属加工液和仔细清洁后，仍观察到快速重新定植。使用铜绿假单胞菌 PAO1 作为模型生物膜生物，棒曲霉素 (40 µM) 和呋喃酮 C-30 (75 µM) 被确定为有效的 QSI 制剂。与未处理的对照相比，两种试剂的功效均显着高于 α-淀粉酶（细胞外聚合物降解酶），并且 MWF 中的生物膜形成分别减少了 63% 和 76%。在用 QS 抑制剂处理的 MWF 中，假定已鉴定的高丝氨酸内酯和喹啉的产量减少，支持了 QSI 对生物膜形成的影响。结果强调了 QSI 作为消除金属加工液中生物膜的潜在策略的有效性。
Microbial contamination in metalworking systems is a critical problem. This study determined the microbial communities in metalworking fluids (MWFs) from two machining shops and investigated the effect of quorum sensing inhibition (QSI) on biofilm growth. In both operations, biofilm-associated and planktonic microbial communities were dominated byPseudomonadales(60.2–99.7%). Rapid recolonization was observed even after dumping spent MWFs and meticulous cleaning. UsingPseudomonas aeruginosaPAO1 as a model biofilm organism, patulin (40 µM) and furanone C-30 (75 µM) were identified as effective QSI agents. Both agents had a substantially higher efficacy compared to α-amylase (extracellular polymeric substance degrading enzyme) and reduced biofilm formation by 63% and 76%, respectively, in MWF when compared to untreated controls. Reduced production of putatively identified homoserine lactones and quinoline in MWF treated with QS inhibitors support the effect of QSI on biofilm formation. The results highlight the effectiveness of QSI as a potential strategy to eradicate biofilms in MWFs.