Follicle-stimulating hormone regulation of estradiol production: Possible involvement of WNT2 and β-catenin in bovine granulosa cells
Follicle-stimulating hormone regulation of estradiol production: Possible involvement of WNT2 and β-catenin in bovine granulosa cells
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DOI：
10.2527/jas.2011-4696
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发表时间：
2012-11-01
影响因子：
3.3
通讯作者：
Gifford, J. A. Hernandez
中科院分区：
文献类型：
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作者：
Castanon, B. I.;Stapp, A. D.;Gifford, J. A. Hernandez
促卵泡激素调节成年啮齿动物卵巢中雌激素的生物合成需要β-连环蛋白（CTNNB 1），但CTNNB 1是否参与FSH诱导的牛雌激素产生尚不清楚。为了阐明FSH在调节特定无翅型小鼠乳腺肿瘤病毒整合位点（WNT）/CTNNB 1途径组分在牛卵泡生成和类固醇生成中的作用，从含有III期黄体（发情周期第11至17天）的卵巢的大窦卵泡（8至22 mm）中收集颗粒细胞和卵泡液。卵泡分为高雌二醇（n = 3; >= 25 ng/mL）或低雌二醇（n = 3;
Follicle-stimulating hormone regulation of estrogen biosynthesis in the adult rodent ovary requires beta-catenin (CTNNB1), but whether CTNNB1 is involved in FSH-induced estrogen production in cattle is unknown. To elucidate the effect of FSH in regulating specific wingless-type mouse mammary tumor virus integration site (WNT)/CTNNB1 pathway components in bovine folliculogenesis and steroidogenesis, granulosa cells and follicular fluid were collected from large antral follicles (8 to 22 mm) from ovaries containing stage-III corpora lutea (d 11 to 17 of an estrous cycle). Follicles were categorized as high estradiol (n = 3; >= 25 ng/mL) or low estradiol (n = 3;