Biofilm formation by Pseudomonas aeruginosa wild type, flagella and type IV pili mutants
Biofilm formation by Pseudomonas aeruginosa wild type, flagella and type IV pili mutants
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DOI：
10.1046/j.1365-2958.2003.03525.x
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发表时间：
2003-06-01
影响因子：
3.6
通讯作者：
Tolker-Nielsen, T
中科院分区：
文献类型：
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作者：
Klausen, M;Heydorn, A;Tolker-Nielsen, T
通过使用共焦激光扫描显微镜和 comstat 图像分析来表征在用柠檬酸盐基本培养基灌溉的流动室中由 Gfp 标记的铜绿假单胞菌 PAO1 野生型、鞭毛和 IV 型菌毛突变体形成的生物膜。鞭毛和 IV 型菌毛对于铜绿假单胞菌初始附着或生物膜形成不是必需的，但细胞附属物在生物膜发育中发挥作用，因为野生型、鞭毛和 IV 型菌毛突变体形成具有不同结构的生物膜。通过用 Yfp 和 Cfp 标记野生型和鞭毛/IV 型突变体并在混合颜色生物膜中进行延时共焦激光扫描显微镜检查，研究了生物膜形成过程中的动力学和选择。最初的小菌落通过克隆生长形成，之后野生型铜绿假单胞菌通过抽搐运动在培养基上扩散。野生型生物膜是动态组合物，在发育过程中具有广泛的运动性、竞争性和选择性。细菌迁移阻止了野生型生物膜中更大的微菌落结构的形成。讨论了与铜绿假单胞菌生物膜发育的当前模型相关的结果。
Biofilm formation by Gfp-tagged Pseudomonas aeruginosa PAO1 wild type, flagella and type IV pili mutants in flow chambers irrigated with citrate minimal medium was characterized by the use of confocal laser scanning microscopy and comstat image analysis. Flagella and type IV pili were not necessary for P. aeruginosa initial attachment or biofilm formation, but the cell appendages had roles in biofilm development, as wild type, flagella and type IV pili mutants formed biofilms with different structures. Dynamics and selection during biofilm formation were investigated by tagging the wild type and flagella/type IV mutants with Yfp and Cfp and performing time-lapse confocal laser scanning microscopy in mixed colour biofilms. The initial microcolony formation occurred by clonal growth, after which wild-type P. aeruginosa bacteria spread over the substratum by means of twitching motility. The wild-type biofilms were dynamic compositions with extensive motility, competition and selection occurring during development. Bacterial migration prevented the formation of larger microcolonial structures in the wild-type biofilms. The results are discussed in relation to the current model for P. aeruginosa biofilm development.