Layer-by-layer assembly of multi-layered droplet interface bilayers (multi-DIBs).
Layer-by-layer assembly of multi-layered droplet interface bilayers (multi-DIBs).
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多层液滴界面双层（multi-DIB）的逐层组装。
DOI：
10.1039/d1cc05155e
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Allen ME
中科院分区：
文献类型：
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作者：
Allen ME
液滴界面双层（DIB）作为基础生物膜研究和生物技术的平台具有巨大的前景。然而，由单个双层组成限制了它们的仿生潜力，因为许多细胞膜基序是由多个对齐的双层组成。我们描述了一种通过用另外的单层涂覆巨大的单层囊泡来制造细胞大小的多层DIB（多DIB）的技术，并允许这种结构与其自身或单层涂覆的液滴接触。这种易于定制的策略将为扩展 DIB 功能铺平道路，例如通过促进多个双层跨越蛋白复合物的掺入。
Droplet interface bilayers (DIBs) have tremendous promise as platforms for fundamental biomembrane studies and in biotechnology. Being composed of a single bilayer however limits their biomimetic potential, as many cell membrane motifs are composed of multiple aligned bilayers. We describe a technology to manufacture cell-sized multi-layered DIBs (multi-DIBs) by coating giant unilamellar vesicles with a further monolayer, and allowing such structures to make contact with themselves or a monolayer coated droplet. This easily customisable strategy will pave the way for an expanded repertoire of DIB functionality, for example by facilitating the incorporation of multiple-bilayer spanning protein complexes.