Evaluation of the Autoanalysis Colilert test for detection and enumeration of total coliforms
Evaluation of the Autoanalysis Colilert test for detection and enumeration of total coliforms
复制标题
用于检测和计数总大肠菌群的 Autoanalysis Colilert 测试的评估
DOI：
--
复制
发表时间：
1989
影响因子：
4.4
通讯作者：
R W Freyberg
中科院分区：
文献类型：
--
作者：
T C Covert;L. Shadix;E W Rice;J R Haines;R W Freyberg
将自动分析Colilert（AC）试验与膜过滤器（MF）、10管多管发酵（MTF）技术以及水和废水检验标准方法中描述的存在-不存在试验进行比较，以检测和计数水中的总大肠菌群。用来自7个不同来源的31个样品对方法进行了评价。采用每种技术分析每份样品，重复使用100 ml样品体积。共有582支确认试管经MTF试验呈阳性，533支试管经AC试验呈阳性。最可能数可比性数据的统计分析显示，阳性试管数量存在统计学显著性差异，MTF检测导致更多阳性试管。两种方法之间的精密度无统计学显著差异。三种方法对总大肠菌群的检测结果具有可比性。一般在饮用水中遇到的异养菌水平不干扰检测或计数的大肠菌群的AC测试。
The Autoanalysis Colilert (AC) test was compared with the membrane filter (MF), 10-tube multiple-tube fermentation (MTF) technique, and the presence-absence test as described in Standard Methods for the Examination of Water and Wastewater for the detection and enumeration of total coliforms in water. The methods were evaluated with 31 samples from seven different sources. Each sample was analyzed by each of the techniques, using replicate 100-ml sample volumes. A total of 582 confirmed tubes were positive by the MTF test, and 533 tubes were positive by the AC test. Statistical analysis of the most-probable-number comparability data showed a statistically significant difference in the number of positive tubes, with the MTF test resulting in more positive tubes. There were no statistically significant differences in precision between the two methods. All the methods were comparable in detection of total coliforms. Levels of heterotrophic bacteria generally encountered in drinking water did not interfere with detection or enumeration of coliforms by the AC test.