Stable L-forms of Clostridium perfringens and their growth on glass surfaces.
Stable L-forms of Clostridium perfringens and their growth on glass surfaces.
复制标题
产气荚膜梭菌的稳定 L 型及其在玻璃表面的生长。
DOI：
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发表时间：
1976
期刊：
影响因子：
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通讯作者：
T. Moore
中科院分区：
文献类型：
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作者：
D. E. Mahony;T. Moore
在含10%蔗糖和2单位青霉素的脑心灌注液中诱导l型产气荚膜梭菌。生长数小时后，可见球质体、颗粒和细长杆菌。每隔24小时，在上述培养基中进行连续传代培养，结果培养完全由球质体（或原生质体）和颗粒组成。在停用青霉素后，这些l型培养物生长良好，经过100次传代后，没有恢复到细菌形式。蔗糖也可以从培养基中提取出来。考察了离心、渗透稳定剂、紫外光、温度、pH和冻干对稳定l型的影响。l型在生长过程中被发现附着在培养管的壁上，l型生长片在莱顿管的盖片上和药瓶的侧面得到。
L-forms of Clostridium perfringens were induced in brain heart infusion broth containing 10% sucrose and 2 units of penicillin. After a few hours of growth, spheroplasts, granules, and elongated bacilli were apparent. At 24-h intervals, serial subcultures were made in the above medium which resulted in a culture composed entirely of spheroplasts (or protoplasts) and granules. Upon the withdrawal of penicillin these L-form cultures grew well and, after 100 passages, there was no reversion to the bacillary form. Sucrose could also be withdrawn from the medium. The effects of centrifugation, osmotic stabilizer, ultraviolet light, temperature, pH, and lyophilization upon stable L-forms were examined. L-forms were found to attach to the walls of culture tubes during trowth and sheets of L-form growth were obtained on cover slips in Leighton tubes and on the sides of medicine bottles.