The rotational order-disorder structure of the reversibly photoswitchable red fluorescent protein rsTagRFP.
The rotational order-disorder structure of the reversibly photoswitchable red fluorescent protein rsTagRFP.
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可逆光开关红色荧光蛋白 rsTagRFP 的旋转有序-无序结构。
DOI：
10.1107/s1399004713024644
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Dauter,Zbigniew
中科院分区：
文献类型：
--
作者：
Pletnev,Sergei;Subach,FedorV;Verkhusha,VladislavV;Dauter,Zbigniew
详细讨论了可逆光开关荧光蛋白rsTagRFP的旋转序-无序结构。该结构是由222个对称性的四聚体以两个不同的取向结合在晶格中，围绕晶体c轴彼此旋转90°，四聚体的轴与晶体学两重轴重合。晶体中交替取向的四异构体的随机分布创造了统计平均的I422对称性的旋转OD结构。尽管有有序无序的病理，rsTagRFP的结构对于模型的非重叠和重叠部分都具有良好的电子密度图。讨论了晶体接触、晶体内部结构和旋转OD晶体形成的可能机制。
The rotational order–disorder (OD) structure of the reversibly photoswitchable fluorescent protein rsTagRFP is discussed in detail. The structure is composed of tetramers of 222 symmetry incorporated into the lattice in two different orientations rotated 90° with respect to each other around the crystal c axis and with tetramer axes coinciding with the crystallographic twofold axes. The random distribution of alternatively oriented tetramers in the crystal creates the rotational OD structure with statistically averaged I422 symmetry. Despite order–disorder pathology, the structure of rsTagRFP has electron-density maps of good quality for both non-overlapping and overlapping parts of the model. The crystal contacts, crystal internal architecture and a possible mechanism of rotational OD crystal formation are discussed.