Detection of highly pathogenic avian influenza virus infection in vaccinated chicken flocks by monitoring antibodies against non-structural protein 1 (NS1)
Detection of highly pathogenic avian influenza virus infection in vaccinated chicken flocks by monitoring antibodies against non-structural protein 1 (NS1)
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DOI：
10.1016/j.vetmic.2010.07.002
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发表时间：
2011-01-27
影响因子：
3.3
通讯作者：
Kida, Hiroshi
中科院分区：
文献类型：
--
作者：
Takeyama, Natsumi;Minari, Kenji;Kida, Hiroshi
H5 和 H7 高致病性禽流感病毒 (HPAIV) 是全球家禽和人类健康的主要关注点。禽流感 (AI) 疫苗是可用的，但不适合现场应用，主要是因为疫苗接种会干扰 AIV 感染的血清监测。为了克服这个问题，开发了使用 AIV 非结构蛋白 1 (NS1) 作为抗原的 ELISA 系统 (NS1-ELISA)，用于测量在感染 AIV 的鸡中产生的抗 NS1 抗体，但在接种疫苗的鸡中不产生这种抗体。然而，一些接种AI疫苗的鸡抗病毒免疫反应较弱，随后可能会感染AIV并传播病毒。这引起了人们对 NS1-ELISA 检测先前接种疫苗的鸡中 AIV 感染的有效性的担忧。在这项研究中，我们开发了 NS1-ELISA 并评估了其在先前接种过 H5 或 H7 AI 疫苗的鸡中检测 HPAIV 感染的可行性。结果表明，NS1-ELISA 可以在感染后 1 周识别未接种疫苗和接种疫苗的鸡中的 HPAIV 感染，与耗时的病毒分离测试结果相关。总而言之，当 AI 疫苗计划到位时，NS1-ELISA 系统将成为定义 HPAIV 感染的宝贵工具。 (C) 2010 Elsevier B.V. 保留所有权利。
H5 and H7 highly pathogenic avian influenza virus (HPAIV) represent a major global concern in poultries and human health. Avian influenza (AI) vaccines are available but not preferred for field applications, primarily because vaccination interferes with sero-surveillances of AIV infection. To overcome the problem, ELISA systems using nonstructural protein 1 (NS1) of AIV as antigens (NS1-ELISA) have been developed to measure anti-NS1 antibodies that are raised in AIV-infected but not in vaccinated chickens. However, some AI-vaccinated chickens having a weak anti-virus immune response may subsequently be infected with AIV and spread the virus. This raises a concern for the validity of NS1-ELISA to detect AIV infection in previously vaccinated chickens. In this study, we developed NS1-ELISA and assessed its feasibility to detect HPAIV infection in chickens previously immunized with H5 or H7 AI vaccines. The results indicated that the NS1-ELISA could identify HPAIV infection in both unvaccinated and vaccinated chickens at 1 week after infection in correlation with results from time-consuming virus isolation tests. Taken together, the NS1-ELISA system would be valuable tool to define HPAIV infection when AI vaccine program is in place. (C) 2010 Elsevier B.V. All rights reserved.