Histidylated cationic polyorganophosphazene/DNA self-assembled nanoparticles for gene delivery.
Histidylated cationic polyorganophosphazene/DNA self-assembled nanoparticles for gene delivery.
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DOI：
10.1016/j.ijpharm.2007.11.041
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发表时间：
2008-04
影响因子：
5.8
通讯作者：
Yongxin Yang;Zhenghong Xu;Shang-wei Chen;Yu Gao;Wangwen Gu;Lingli Chen;Y. Pei;Yaping Li
中科院分区：
文献类型：
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作者：
Yongxin Yang;Zhenghong Xu;Shang-wei Chen;Yu Gao;Wangwen Gu;Lingli Chen;Y. Pei;Yaping Li
Cationic polyorganophosphazene has shown the ability to deliver gene. To obtain more efficient transfection, His(Boc)-OMe bearing histidine moiety was introduced to synthesize a new derivative of cationic polyphosphazenes with another side group of 2-dimethylaminoethylamine (DMAEA). The poly(DMAEA/His(Boc)-OMe)phosphazene (PDHP) and DNA could self-assemble into nanoparticles with a size around 110nm and zeta potential of +15mV at the PDHP/DNA ratio of 10:1 (w/w). The maximum transfection efficiency of PDHP/DNA self-assembled nanoparticles (PHSNs) against 293 T cells was much higher than that of poly(di-2-dimethylaminoethylamine) phosphazenes (PDAP)/DNA self-assembled nanoparticles (PASNs) and PEI 25/DNA self-assembled nanoparticles (PESNs) at the polymer/DNA ratio of 10:1, but the cytotoxicity of PDHP assayed by MTT was much lower than that of PDAP and PEI 25. These results suggested that PDHP could be a good candidate with high transfection efficiency and low cytotoxicity for gene delivery.
