Identification of 13 Spirogyra Species (Zygnemataceae) by Traits of Sexual Reproduction Induced Under Laboratory Culture Conditions
Identification of 13 Spirogyra Species (Zygnemataceae) by Traits of Sexual Reproduction Induced Under Laboratory Culture Conditions
复制标题
通过实验室培养条件下诱导的有性生殖特征鉴定 13 种水绵（水绵科）
DOI：
10.1038/s41598-019-43454-6
复制
发表时间：
2019
影响因子：
4.6
通讯作者：
Nozaki H
中科院分区：
文献类型：
--
作者：
Takano T;Higuchi S;Ikegaya H;Matsuzaki R;Kawachi K;Takahashi F;Nozaki H
水绵属在世界各地的淡水栖息地丰富，包括约380种。由于鉴定是基于野外采集的样品中有性生殖的特征和野外或实验室培养中产生的孢子，因此物种分配通常很困难。我们开发了一个鉴定程序的基础上，一个改进的方法，诱导性接合在实验室培养的花丝。我们测试了52个新成立的和遗传不同的菌株收集来自不同的地方在日本的修改程序。我们在受控的实验室条件下，在52个菌株中的15个菌株中诱导接合或形成游动孢子，这使我们能够识别13个物种。根据有性生殖的独特特征，13个物种中的两个可归属于一个相关但分类学上不确定的属Temnogyra。我们的系统发育分析表明，两个Temnogyraspecies包括在一个大的分支，包括许多种水绵。因此，将Temnogyra与Spirogyra分离可能是站不住脚的，因为分子分析不支持将Sirogonium与Spirogyra分离。
The genusSpirogyrais abundant in freshwater habitats worldwide, and comprises approximately 380 species. Species assignment is often difficult because identification is based on the characteristics of sexual reproduction in wild-collected samples and spores produced in the field or laboratory culture. We developed an identification procedure based on an improved methodology for inducing sexual conjugation in laboratory-cultivated filaments. We tested the modified procedure on 52 newly established and genetically different strains collected from diverse localities in Japan. We induced conjugation or aplanospore formation under controlled laboratory conditions in 15 of the 52 strains, which allowed us to identify 13 species. Two of the thirteen species were assignable to a related but taxonomically uncertain genus,Temnogyra, based on the unique characteristics of sexual reproduction. Our phylogenetic analysis demonstrated that the twoTemnogyraspecies are included in a large clade comprising many species ofSpirogyra. Thus, separation ofTemnogyrafromSpirogyramay be untenable, much as the separation ofSirogoniumfromSpirogyrais not supported by molecular analyses.