GAS-EXCHANGE AND LUNG MORPHOLOGY AFTER SURFACTANT REPLACEMENT IN EXPERIMENTAL ADULT RESPIRATORY-DISTRESS SYNDROME INDUCED BY REPEATED LUNG LAVAGE
GAS-EXCHANGE AND LUNG MORPHOLOGY AFTER SURFACTANT REPLACEMENT IN EXPERIMENTAL ADULT RESPIRATORY-DISTRESS SYNDROME INDUCED BY REPEATED LUNG LAVAGE
复制标题
DOI：
10.1111/j.1399-6576.1986.tb02423.x
复制
发表时间：
1986-05-01
影响因子：
2.1
通讯作者：
ROBERTSON, B
中科院分区：
文献类型：
--
作者：
BERGGREN, P;LACHMANN, B;ROBERTSON, B
通过反复肺灌洗在成年豚鼠中诱导严重呼吸功能不全。然后动物在100%O2、吹入压力28/6-8 cmH 2 O（2.7/0.6-0.8 kPa）、频率30/min和33%吸气时间下通气75 min。一组动物（I）用去除蛋白质的猪表面活性剂处理，所述表面活性剂通过蔗糖梯度离心、加热至90 ℃、C和氯仿/甲醇萃取。另一组（II）收到的磷脂馏分的猪表面活性剂，分离从切碎的肺氯仿/甲醇提取和液体凝胶色谱法。在灌洗程序后约15和45分钟，通过气管插管以两个1 ml剂量（脂质浓度90 mg/ml）滴注表面活性剂。未处理的灌洗动物作为对照。通气75分钟后，PaO 2和PaCO 2的对照值为13.3 ± 0.01。6.8和6.8 .+-。2.3 kPa（平均值±）s.d.），分别经表面活性剂处理的第I组动物的相应值为52.9 ±。7.7和4.4 .+-。1.1Ⅱ组为53.5 ±. kPa。7.3和4.8 .+-。1.3 kPa（P < 0.02-0.002）。在组织切片中，两组经表面活性剂处理的动物也具有显著改善的肺泡空气膨胀，这反映在肺泡体积密度增加（0.67 ± 0.95）。0.05和0.62 ±。0.11相对于0.45 ±。0.08对照组P < 0.002）。因此，该实验模型中表面活性剂替代的益处与先前在新生儿表面活性剂缺乏症动物模型以及呼吸窘迫综合征（RDS）婴儿中观察到的益处相似。我们的数据表明，表面活性物质的替代可能有治疗效果，也在临床成人RDS。
Severe respiratory insufficiency was induced in adult guinea pigs by repeated lung lavage. The animals were then ventilated for 75 min with 100% O2, insufflation pressure 28/6-8 cmH2O (2.7/0.6-0.8 kPa), frequency 30/min, and 33% inspiration time. One group of animals (I) was treated with protein-depleted porcine surfactants, prepared by a combination of sucrose-gradient centrifugation, heating to 90.degree. C, and chloroform/methanol extraction. Another group (II) received the phospholipid fraction of porcine surfactants, isolated from minced lungs by chloroform/methanol extraction and liquid-gel chromatography. Surfactant was administered in two 1-ml doses (lipid concentration 90 mg/ml) instilled via the tracheal cannula about 15 and 45 min after the lavage procedure. Non-treated, lavaged animals served as controls. After 75 min of ventilation, control values for PaO2 and PaCO2 were 13.3 .+-. 6.8 and 6.8 .+-. 2.3 kPa (mean .+-. s.d.), respectively. The corresponding values in Group I of surfactant-treated animals were 52.9 .+-. 7.7 and 4.4 .+-. 1.1 kPa, in Group II 53.5 .+-. 7.3 and 4.8 .+-. 1.3 kPa (P < 0.02-0.002). The two groups of surfactant-treated animals also had significantly improved alveolar air expansion in histological sections, as reflected by increased alveolar volume density (0.67 .+-. 0.05 and 0.62 .+-. 0.11 vs. 0.45 .+-. 0.08 in controls; P < 0.002). The benefits of surfactant replacement in this experimental model were thus similar to those previously observed in animal models of neonatal surfactant deficiency as well as in babies with respiratory distress syndrome (RDS). Our data suggest that surfactant replacement might have a therapeutic effect also in clinical adult RDS.