Hydrogen peroxide producing lactobacilli in women with vaginal infections
Hydrogen peroxide producing lactobacilli in women with vaginal infections
复制标题
DOI：
10.1016/j.ejogrb.2005.11.036
复制
发表时间：
2006-11-01
影响因子：
2.6
通讯作者：
Ranin, Lazar T.
中科院分区：
文献类型：
--
作者：
Mijac, Vera D.;Dukic, Slobodanka V.;Ranin, Lazar T.
目的：产过氧化氢乳酸杆菌是从大多数健康育龄妇女阴道中分离出来的。它们对阴道中病原体过度生长的毒性和抑制作用已通过体外研究证明。关于产生过氧化氢的乳酸杆菌在阴道感染中的作用的临床研究是有争议的。研究设计：本研究的目的是从患有主要阴道感染的妇女中分离乳酸杆菌：细菌性阴道病（BV）、外阴阴道念珠菌病（VVC）和滴虫病（TV），并来自具有正常植物群（NF）的妇女，以测试其产生过氧化氢的能力，定量乳酸杆菌并评估与产生过氧化氢的乳酸杆菌在阴道定植相关的因素。研究组包括108名妇女：27名VVC，26名BV，20名TV和35名NF。采用Fisher精确检验、卡方检验和方差分析对数据进行分析。81.5%的VVC女性和58%的BV女性中分离出乳杆菌（p = 0019），55%的女性有电视（p = 0.015）和86%的NE女性。来自VVC、TV和NF女性的大多数乳酸杆菌分离株产生过氧化氢（分别为77%、63.5%和80%），而BV妇女只有26.5%的分离株产生过氧化氢（p = 0.01）。定量分析表明，在绝大多数妇女与乳酸杆菌定殖，其计数>10（7）CFU/ ml。在对行为和人口统计学特征进行评估后，我们发现吸烟者分离出产生过氧化氢的乳酸杆菌的发生率较低（p = 0.018）。我们的研究结果表明，女性BV缺乏乳酸杆菌，特别是过氧化氢生产的。有电视的女性乳酸杆菌的比例较低。在VVC的妇女，既没有显着差异，在分离的乳酸杆菌，也没有在他们的能力，产生过氧化氢，与NF的妇女相比。结论：我们的研究结果指出，产生过氧化氢的乳酸杆菌可以防止BV的发展，但不能防止VVC和TV。(C)2006年由Elsevier爱尔兰有限公司出版。
Objective: Hydrogen peroxide producing lactobacilli are isolated from the vaginas of a majority of healthy reproductive age women. Their toxic and inhibitory effect against the overgrowth of pathogens in the vagina is documented by in vitro studies. Clinical studies concerning the role of hydrogen peroxide producing lactobacilli in vaginal infections are controversial.Study design: The aim of this study was to isolate lactobacilli from women with major vaginal infections: bacterial vaginosis (BV), vulvovaginal candidiasis (VVC) and trichomoniasis (TV), and from women with normal flora (NF), to test their ability to produce hydrogen peroxide, to quantitate lactobacilli and to evaluate factors related to the colonization of vagina with hydrogen peroxide producing lactobacilli. The study group comprised 108 women: 27 with VVC, 26 with BV, 20 with TV and 35 with NF. Data was analysed with Fisher exact test, chi(2)- test, and ANOVA.Results: Lactobacilli were isolated from 81.5% of women with VVC, 58% of women with BV (p = 0019), 55% of women with TV (p = 0.015) and from 86% of women with NE The majority of isolates of lactobacilli from women with VVC, TV and NF produced hydrogen peroxide (77%, 63.5% and 80%, respectively), compared with women with BV where only 26.5% of isolates produced hydrogen peroxide (p = 0.01). Quantitative analysis showed that in an overwhelming majority of women colonized with lactobacilli, their count was >10(7) CFU/ ml. Upon evaluation of behavioural and demographic characteristics, we found that smokers had a lower incidence of isolation of hydrogen peroxide producing lactobacilli (p = 0.018). Our results showed that women with BV lack lactobacilli, especially hydrogen peroxide producing ones. Women with TV had a lower rate of lactobacilli. In women with VVC, there was neither significant difference in the isolation of lactobacilli, nor in their ability to produce hydrogen peroxide, compared to women with NF.Conclusion: Our results point out that hydrogen peroxide producing lactobacilli could protect against the development of BV, but not against VVC and TV. (C) 2006 Published by Elsevier Ireland Ltd.