Label-Free and High-Throughput Detection of Biomolecular Interactions Using a Flatbed Scanner Biosensor
Label-Free and High-Throughput Detection of Biomolecular Interactions Using a Flatbed Scanner Biosensor
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DOI：
10.1021/acssensors.7b00263
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发表时间：
2017-10-01
期刊：
影响因子：
8.9
通讯作者：
Ozkumur, Ayca Yalcin
中科院分区：
文献类型：
--
作者：
Aygun, Ugur;Avci, Oguzhan;Ozkumur, Ayca Yalcin
基于荧光的微阵列检测系统提供灵敏的测量;然而，探针固定的变化和差的可重复性负面地影响最终的读出，并因此影响这些系统的定量能力。在这里，我们展示了一个无标记和高通量的光学生物传感器，可用于校准的荧光微阵列。该传感器采用了商业平板扫描仪，我们展示了这种低成本（类似于100美元）的系统转换成干涉反射成像传感器，通过硬件和软件的修改。使用该传感器，我们报告了在无标记微阵列上检测DNA杂交和DNA定向抗体固定，其噪声基底类似于30 pg/mm（2），扫描速度为5 s（10帧平均50 s），面积为2 mm X 2 mm。这种新的系统可用作独立的无标记传感器，特别是在低资源环境中，以及用于现有基于荧光的DNA和蛋白质检测平台中的微阵列的质量控制和校准。
Fluorescence based microarray detection systems provide sensitive measurements; however, variation of probe immobilization and poor repeatability negatively affect the final readout, and thus quantification capability of these systems. Here, we demonstrate a label-free and high-throughput optical biosensor that can be utilized for calibration of fluorescence microarrays. The sensor employs a commercial flatbed scanner, and we demonstrate transformation of this low cost (similar to 100 USD) system into an Interferometric Reflectance Imaging Sensor through hardware and software modifications. Using this sensor, we report detection of DNA hybridization and DNA directed antibody immobilization on label-free microarrays with a noise floor of similar to 30 pg/mm(2), and a scan speed of 5 s (50 s for 10 frames averaged) for a 2 mm X 2 mm area. This novel system may be used as a standalone label-free sensor especially in low-resource settings, as well as for quality control and calibration of microarrays in existing fluorescence-based DNA and protein detection platforms.