Microglia-specific localisation of a novel calcium binding protein, Iba1
Microglia-specific localisation of a novel calcium binding protein, Iba1
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DOI：
10.1016/s0169-328x(98)00040-0
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发表时间：
1998-06-01
期刊：
影响因子：
--
通讯作者：
Kohsaka, S
中科院分区：
文献类型：
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作者：
Ito, D;Imai, Y;Kohsaka, S
Iba1 （ionized calcium binding adaptor molecule 1）是本课题组克隆的一种新型钙结合蛋白cdna，其mRNA在大鼠脑细胞培养的小胶质细胞中特异性表达[Imai等]。Biophys。物化学。Commun >,， 24(1996) 855-862]。在本研究中，免疫细胞化学和免疫组织化学检查表明，Iba1蛋白分别在培养的脑细胞和大脑中的小胶质细胞中单独表达。在胚胎大鼠脑混合细胞培养中，小胶质细胞免疫细胞化学阳性，但在神经元、星形胶质细胞或少突胶质细胞中未检测到Iba1蛋白。成年大鼠脑切片免疫组化染色显示Iba1蛋白特异性定位于分枝小胶质细胞。此外，对面神经剪断后面神经核活化小胶质细胞进行免疫组化染色和免疫印迹分析，发现Iba1蛋白表达上调，并在第7天达到峰值。这些结果表明，Iba1蛋白在体外和体内均局限于小胶质细胞，并且Iba1蛋白在调节小胶质细胞的功能中起作用，特别是在活化的小胶质细胞中。(C) 1998 Elsevier Science B.V.版权所有
Recently it has been shown that mRNA of Iba1 (ionized calcium binding adaptor molecule 1), which was a novel calcium binding protein cDNA-cloned by our group, is specifically expressed in microglia in cultures of rat brain cells [Imai et al. Biophys. Biochem. Res. Commun., 224 (1996) 855-862]. In the present study, immunocytochemical and immunohistochemical examinations demonstrated that Iba1 protein is expressed in microglia alone both in cultured brain cells and in the brain, respectively. In a mixed cell culture of embryonic rat brain, immunocytochemically positive for Iba1 protein were the microglia but it was not detectable in neurons, astroglia, or oligodendroglia. Immunohistochemical staining of adult rat brain sections showed Iba1 protein to be specifically localised in ramified microglia. In addition, immunohistochemical staining and immunoblot analysis of activated microglia in the facial nucleus after facial nerve axotomy shows that expression of Iba1 protein was upregulated and peaked at 7 days. These results indicated that localisation of Iba1 protein is restricted to microglia both in vitro and in vivo, and that Iba1 protein plays a role in regulating the function of microglia, especially in the activated microglia. (C) 1998 Elsevier Science B.V. All rights reserved.