Disruption of spermatogenesis and differential regulation of testicular estrogen receptor expression in mice after polychlorinated biphenyl exposure
Disruption of spermatogenesis and differential regulation of testicular estrogen receptor expression in mice after polychlorinated biphenyl exposure
复制标题
DOI：
10.1016/j.tox.2011.05.010
复制
发表时间：
2011-09-05
期刊：
影响因子：
4.5
通讯作者：
Zuo, Zhenghong
中科院分区：
文献类型：
--
作者：
Cai, Jiali;Wang, Chonggang;Zuo, Zhenghong
多氯联苯 (PCB) 的睾丸毒性已得到广泛研究。然而，详细机制仍不清楚。在本研究中，雄性 C57 小鼠每 3 天通过口服管饲法接受不同剂量的 Aroclor 1254（一种市售 PCB 混合物）。暴露于 Aroclor 1254 50 天后，精子数量以剂量依赖性方式下降。细胞增殖和凋亡是调节睾丸发育的关键过程，这些过程的改变可能是睾丸发育不全的原因。我们的结果显示，Aroclor 1254 治疗后，生殖细胞增殖受到抑制，并以剂量依赖性方式诱导生殖细胞凋亡。尽管不同剂量的 Aroclor 1254 治疗后，血清睾酮水平和雄激素受体表达水平没有显着变化，但雌二醇水平下降，雌激素受体（ER）β 表达呈剂量依赖性增加，而 ER α 表达仅在50μg/kg组。整体数据表明，抑制生殖细胞增殖并诱导细胞凋亡以及 ER 的差异调节可能与 PCB 的睾丸毒性有关。 (C) 2011 Elsevier Ireland Ltd. 保留所有权利。
The testicular toxicity of polychlorinated biphenyls (PCBs) has been extensively studied. However, the detailed mechanism is still obscure. In the present study, male C57 mice were treated with different doses of Aroclor 1254 (a commercial PCB mixture) once every 3 days by oral gavage. After exposure to Aroclor 1254 for 50 days, the sperm count decreased in a dose-dependent manner. Cell proliferation and apoptosis are key processes regulating development of the testis, and alterations in these processes may underlie testicular dysgenesis. Our results showed that the germ cell proliferation was inhibited and the apoptosis of the germ cell was induced in a dose-dependent manner after treatment with Aroclor 1254. Although there was no significant change in serum testosterone levels and androgen receptor expression levels after treatment with different dosages of Aroclor 1254, the estradiol levels decreased and the expression of estrogen receptor (ER) beta increased in a dose-dependent manner, whereas an elevation of the expression of ER alpha was only observed in the 50 mu g/kg group. The data as a whole suggested that inhibited proliferation and induced apoptosis in germ cells, and a differential regulation of ER, may be involved in the testicular toxicity of PCBs. (C) 2011 Elsevier Ireland Ltd. All rights reserved.