Targeted delivery of vascular endothelial growth factor improves stem cell therapy in a rat myocardial infarction model.
Targeted delivery of vascular endothelial growth factor improves stem cell therapy in a rat myocardial infarction model.
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DOI：
10.1016/j.nano.2014.06.001
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发表时间：
2014-11
期刊：
影响因子：
--
通讯作者：
Wang B
中科院分区：
文献类型：
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作者：
Tang Y;Gan X;Cheheltani R;Curran E;Lamberti G;Krynska B;Kiani MF;Wang B
通过间充质干细胞（MSC）重建梗死心肌尚未成功，因为部分由于心肌梗死（MI）后血液供应不足而导致细胞存活不良。我们假设，有针对性地交付的血管内皮生长因子（VEGF）心肌梗死可以帮助再生血管，支持MSC治疗大鼠模型的心肌梗死。心肌梗死后立即通过尾静脉输注VEGF包裹的免疫脂质体，靶向心肌梗死组织中过表达的P-选择素。一周后，心肌内注射MSCs。通过靶向递送VEGF或MSC治疗，心脏功能损失略微缓和，而靶向VEGF + MSC组合治疗显示心脏功能损失的最高衰减。联合治疗还显著增加了MI后组织中的血管密度（80%）并降低了胶原蛋白含量（33%）。联合治疗组MSCs的植入显著增加，植入的细胞有助于血管的修复。
Rebuilding of infarcted myocardium by mesenchymal stem cells (MSCs) has not been successful because of poor cell survival due in part to insufficient blood supply after myocardial infarction (MI). We hypothesize that targeted delivery of vascular endothelial growth factor (VEGF) to MI can help regenerate vasculature in support of MSC therapy in a rat model of MI. VEGF-encapsulated immunoliposomes targeting overexpressed P-selectin in MI tissue were infused by tail vein immediately after MI. One week later, MSCs were injected intramyocardially. The cardiac function loss was moderated slightly by targeted delivery of VEGF or MSC treatment, while targeted VEGF + MSC combination treatment showed highest attenuation in cardiac function loss. The combination treatment also markedly increased blood vessel density (80%) and decreased the collagen content in post-MI tissue (33%). Engraftment of MSCs in the combination treatment group was significantly increased and the engrafted cells contributed to the restoration of blood vessels.