Influence of norbixin on plasma cholesterol-associated lipoproteins, plasma arylesterase/paraoxonase activity and hepatic lipid peroxidation of Swiss mice on a high fat diet
Influence of norbixin on plasma cholesterol-associated lipoproteins, plasma arylesterase/paraoxonase activity and hepatic lipid peroxidation of Swiss mice on a high fat diet
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DOI：
10.1016/s0308-8146(01)00363-6
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发表时间：
2002-06-01
期刊：
影响因子：
8.8
通讯作者：
Kovary, K
中科院分区：
文献类型：
--
作者：
Santos, AAA;Silva, MVG;Kovary, K
在用高脂肪饮食喂养4周的雄性瑞士小鼠中研究了添加到饮用水中的类胡萝卜素降胭脂树橙（7.7和92.8 mg/kg）的作用。高脂饮食使高密度脂蛋白胆固醇（HDL-cholesterol，P < 0.05）降低55.7%，极低密度脂蛋白胆固醇（VLDL-cholesterol，P < 0.0001）/低密度脂蛋白胆固醇（LDL-cholesterol，P < 0.0001）升高167%。当摄入高脂饮食与降胭脂树橙，HDL-胆固醇和VLDL/LDL-胆固醇保持接近控制水平，而血浆甘油三酯没有改变。高脂饮食使血浆芳基酯酶/对氧磷酶（EC 3.1.8.1）活性降低46%（P < 0.0001），这种降低也被降胭脂树橙抵消。在肝匀浆中测得的硫代巴比妥酸反应物质（TBARS）的基础水平并未因高脂饮食而增加。然而，当来自提交给高脂肪饮食的小鼠的肝匀浆与Fe 2+和H2 O2孵育时，TBARS水平显著增加（P < 0.001）。在相同的氧化条件下，高脂饲料中添加降胭脂树橙时，肝匀浆对过氧化反应的敏感性显著降低（P < 0.0001）。因此，降胭脂树橙，一种常见的类胡萝卜素食品添加剂，改变了瑞士小鼠的血浆脂质水平和对氧磷酶活性与动脉粥样硬化饮食喂养。应进行进一步的研究，以确定最低有效的降胭脂树橙浓度，因为这种类胡萝卜素是非常微量的工业化食品摄入。(C)2002爱思唯尔科技有限公司版权所有。
Effects of the carotenoid norbixin (7.7 and 92.8 mg/kg) added to drinking water were studied in male Swiss mice fed with a high fat diet for 4 weeks. High fat diet decreased, by 55.7%, the levels of high-density lipoprotein (HDL)-cholesterol (P < 0.05) increased, by 167%, the levels of very low-density lipoprotein (VLDL)-cholesterol/low-density lipoprotein (LDL)-cholesterol (P < 0.0001). When the high fat diet was ingested with norbixin, HDL-cholesterol and VLDL/LDL-cholesterol remained near control levels while plasma triglycerides were not altered. High fat diet decreased the activity of plasma arylesterase/paraoxonase (EC3.1.8.1) by 46% (P < 0.0001), a reduction that was also counteracted by norbixin. Basal levels of thiobarbituric acid-reactive substances (TBARS), measured in liver homogenates, were not increased by high fat diet. However, there was a significant increase in TBARS levels (P < 0.001) when liver homogenate from mice submitted to high fat diet was incubated with Fe2+ and H2O2. Under the same oxidative conditions, liver homogenate was much less responsive to peroxidation (P < 0.0001) when high fat diet was associated with norbixin. Thus, norbixin, a common carotenoid food additive, alters plasma lipid levels and paraoxonase activities in Swiss mice fed with an atherogenic diet. Further studies should be performed in order to determine the lowest effective norbixin concentration as this carotenoid is ingested with industrialized food in very tiny amounts. (C) 2002 Elsevier Science Ltd. All rights reserved.