Quantification of the oxygen uptake rate in a dissolved oxygen controlled oscillating jet-driven microbioreactor.
Quantification of the oxygen uptake rate in a dissolved oxygen controlled oscillating jet-driven microbioreactor.
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DOI：
10.1002/jctb.4833
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发表时间：
2016-03
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影响因子：
--
通讯作者：
Szita N
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文献类型：
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作者：
Kirk TV;Marques MP;Radhakrishnan AN;Szita N
微生物反应器已经成为早期生物过程开发的一种新工具。在过去的十年中，这项技术发展迅速，获得过程变量的实时定量数据是当今最先进的技术。此外，还实现了对过程变量的控制。这项研究的目的是建立一种能够控制溶解氧(DO)浓度并实时测定摄氧率的微生物反应器。研制了一种不含任何运动部件的振荡射流驱动膜曝气微生物反应器。获得了∼7 S的混合次数和∼170 h−1的Kla值。DO控制是通过改变电磁微阀的占空比来实现的，从而改变了反应器孵化室中的混合气体。该微生物反应器支持酿酒酵母生长30h以上，菌体密度为6.7mmolgdcw L−1。获得了∼34mmol.L −1h−1的摄氧率。这些结果突出了DO控制的微生物反应器获得关于摄氧率的实时信息的潜力，进而在广泛的处理条件下获得关于各种细胞类型的细胞代谢的实时信息。©2015作者。《化学技术与生物技术》杂志由John Wiley&Sons Ltd代表化学工业学会出版。
Microbioreactors have emerged as a new tool for early bioprocess development. The technology has advanced rapidly in the last decade and obtaining real‐time quantitative data of process variables is nowadays state of the art. In addition, control over process variables has also been achieved. The aim of this study was to build a microbioreactor capable of controlling dissolved oxygen (DO) concentrations and to determine oxygen uptake rate in real time. An oscillating jet driven, membrane‐aerated microbioreactor was developed without comprising any moving parts. Mixing times of ∼7 s, and kLa values of ∼170 h−1 were achieved. DO control was achieved by varying the duty cycle of a solenoid microvalve, which changed the gas mixture in the reactor incubator chamber. The microbioreactor supported Saccharomyces cerevisiae growth over 30 h and cell densities of 6.7 gdcw L−1. Oxygen uptake rates of ∼34 mmol L−1 h−1 were achieved. The results highlight the potential of DO‐controlled microbioreactors to obtain real‐time information on oxygen uptake rate, and by extension on cellular metabolism for a variety of cell types over a broad range of processing conditions. © 2015 The Authors. Journal of Chemical Technology & Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.