BIOSYNTHESIS RATES AND CONTENT OF THYMOSIN-BETA-4 IN CELL-LINES
BIOSYNTHESIS RATES AND CONTENT OF THYMOSIN-BETA-4 IN CELL-LINES
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DOI：
10.1016/0003-9861(85)90028-1
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发表时间：
1985-01-01
影响因子：
3.9
通讯作者：
LEIBOLD, W
中科院分区：
文献类型：
--
作者：
HANNAPPEL, E;LEIBOLD, W
在28种不同的细胞系中测定了胸腺素β4的含量和相对生物合成速率。在 Epstein-Barr 病毒转化的人 B 细胞系中观察到胸腺肽 β4 的最高含量以及最高的生物合成速率。在这些细胞中观察到的水平为每个细胞1pg胸腺素β4，这比大鼠腹膜巨噬细胞中的水平高3倍。因此，这些B细胞系具有迄今为止所描述的任何细胞类型中最高含量的胸腺素β4。由于本文描述的所有 Epstein-Barr 病毒转化的 B 细胞都在悬浮液中生长，因此胸腺素 β4 的存在不太可能与这些细胞中的锚定有关。培养物中活的EB病毒转化的B细胞不分泌胸腺素β4，这表明该肽具有某些细胞内功能。这些B细胞系可能适合研究胸腺肽.BETA.4功能。
The content and relative biosynthetic rates of thymosin .beta.4 was determined in 28 different cell lines. The highest content of thymosin .beta.4 as well as the highest rate of biosynthesis was observed in Epstein-Barr virus-transformed human B-cell lines. The levels observed in these cells are 1 pg thymosin .beta.4 per cell, which is 3 times higher than that in rat peritoneal macrophages. Thus, these B-cell lines have the highest content of thymosin .beta.4 of any cell type yet described. Since all of the Epstein-Barr virus-transformed B-cells described here grow in suspension, it is unlikely that the presence of thymosin .beta.4 is related to anchorage in these cells. Thymosin .beta.4 is not secreted by viable Epstein-Barr virus-transformed B cells in culture, suggesting some intracellular function of the peptide. These B-cell lines may be suitable for the study of thymosin .BETA.4 function.