Trafficking of neutrophils across airway epithelium is dependent upon both thioredoxin‐ and pertussis toxin‐sensitive signaling mechanisms
Trafficking of neutrophils across airway epithelium is dependent upon both thioredoxin‐ and pertussis toxin‐sensitive signaling mechanisms
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中性粒细胞穿过气道上皮的运输依赖于硫氧还蛋白和百日咳毒素敏感的信号机制
作者：
L. Miller;J. Usachenko;R. Mcdonald;D. Hyde
白细胞从循环募集到气道是一个多步骤过程，涉及趋化和粘附机制。使用白细胞跨上皮运输的体外模型，我们发现人外周血中性粒细胞（PMN）沿最佳基底外侧至顶端表面方向跨气道上皮的运动部分被百日咳毒素（Gαi蛋白连接受体的抑制剂）阻断。抗白细胞介素-8（IL-8;气道上皮组成型表达）的中和性单克隆抗体不抑制PMN跨上皮迁移，表明替代性百日咳毒素敏感信号传导机制参与了这一过程。然而，抗硫氧还蛋白（一种具有百日咳毒素不敏感化学引诱物活性的氧化还原酶）的中和抗体确实减少了PMN穿过气道上皮的迁移。我们得出结论，PMN通过气道上皮的运输是由硫氧还蛋白和百日咳毒素敏感的信号传导机制介导的，这些机制不依赖于IL-8。
Leukocyte recruitment from the circulation into the airways is a multi‐step process, involving both chemotactic and adhesive mechanisms. Using an in vitro model of leukocyte transepithelial trafficking, we show that movement of human peripheral blood neutrophils (PMN) across airway epithelium in the optimal basolateral‐to‐apical surface direction is partially blocked by pertussis toxin, an inhibitor of Gαi‐protein‐linked receptors. A neutralizing monoclonal antibody against interleukin‐8 (IL‐8; constitutively expressed by airway epithelium) did not inhibit PMN transepithelial migration, suggesting that alternative pertussis toxin‐sensitive signaling mechanisms are involved in this process. However, a neutralizing antibody against thioredoxin, a redox enzyme with pertussis toxin‐insensitive chemoattractant activity, did reduce PMN migration across airway epithelium. We conclude that trafficking of PMN across airway epithelium is mediated by both thioredoxin‐ and pertussis toxin‐sensitive signaling mechanisms that are independent of IL‐8.
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DOI：
10.1073/pnas.88.17.7538
发表时间：
1991-09-01
影响因子：
11.1
作者：
VONANDRIAN, UH;CHAMBERS, JD;BUTCHER, EC
通讯作者：
BUTCHER, EC
百日咳毒素抑制淋巴细胞和中性粒细胞趋化性。
DOI：
--
发表时间：
1985
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Spangrude,GJ;Sacchi,F;Hill,HR;VanEpps,DE;Daynes,RA
通讯作者：
Daynes,RA
DOI：
10.1164/ajrccm.154.3.8810593
发表时间：
1996-09-01
影响因子：
24.7
作者：
Goodman, RB;Strieter, RM;Martin, TR
通讯作者：
Martin, TR
影响因子：
15.9
作者：
KELLY, CP;BECKER, S;POTHOULAKIS, C
通讯作者：
POTHOULAKIS, C
DOI：
10.1152/ajplung.1999.276.3.l530
发表时间：
1999-03-01
影响因子：
4.9
作者：
Das, KC;Guo, XL;White, CW
通讯作者：
White, CW