A Luciferase-Like Monooxygenase and Flavin Reductase Pair AbmE2/AbmZ Catalyzes Baeyer-Villiger Oxidation in Neoabyssomicin Biosynthesis
A Luciferase-Like Monooxygenase and Flavin Reductase Pair AbmE2/AbmZ Catalyzes Baeyer-Villiger Oxidation in Neoabyssomicin Biosynthesis
复制标题
类荧光素酶单加氧酶和黄素还原酶对 AbmE2/AbmZ 催化新阿比索米星生物合成中的 Baeyer-Villiger 氧化
DOI：
10.1021/acscatal.9b05488
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发表时间：
2020-02-21
期刊：
影响因子：
12.9
通讯作者：
Ju, Jianhua
中科院分区：
文献类型：
--
作者：
Ji, Xiaoqi;Tu, Jiajia;Ju, Jianhua
Baeyer-Villiger monooxygenases (BVMOs) play important roles in the biosynthesis of natural products. We describe here in vivo gene inactivation/complementation studies as well as in vitro enzyme assays that reveal the bacterial luciferase-like monooxgenase (LLM) AbmE2 and its flavin reductase partner AbmZ as a type II BVMO pair that catalyzes BV oxidation during neoabyssomicin assembly in Streptomyces koyangensis SCSIO 5802. This study provides direct biochemical evidence of an LLM that catalyzes BV oxidation.
