Fascin expression persists with fibronectin in embryonic rat hepatoblasts
Fascin expression persists with fibronectin in embryonic rat hepatoblasts
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DOI：
10.1007/s00795-021-00311-8
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发表时间：
2022-01-20
影响因子：
1.8
通讯作者：
Murakami，Ichiro
中科院分区：
文献类型：
--
作者：
Hayashi，Yoshihiro;Yamamoto，Yumiko;Murakami，Ichiro
众所周知，筋膜蛋白和纤维连接蛋白在细胞粘附和迁移中起重要作用。它们被认为是肿瘤标志物或肿瘤治疗的抑制靶点。本研究以大鼠胚胎肝脏为研究对象，检测了肝发育过程中束状蛋白和纤维连接蛋白的表达。然后观察纤维连接蛋白对筋膜蛋白表达的影响。在胚胎日10.5时，当前肠内胚层开始形成肝芽并向横隔扩散时，在肝芽与横隔融合的间隙中观察到纤维状的细胞外基质。在ED11.5时，在纤维连接蛋白阳性的肝母细胞周围观察到纤维连接蛋白。ED13.5时，造血细胞出现，纤维连接蛋白和筋膜蛋白的表达开始下降。在ed14.5时，筋膜蛋白和纤维连接蛋白暂时出现并消失。他们的表情按时间顺序是同步的。随后，通过培养从ED13.5大鼠肝脏分离的肝母细胞来检测纤维连接蛋白对筋膜蛋白的影响。结果，在大多数肝母细胞中，有纤维连接蛋白时，fasin呈阳性，而没有纤维连接蛋白时，fasin的表达明显下降。目前，关于筋膜蛋白与纤维连接蛋白之间关系的研究较少。我们的研究结果表明，纤维连接蛋白可以调节大鼠肝母细胞中筋膜蛋白的表达。
Both fascin and fibronectin are known to play important roles in cell adhesion and migration. They are noted as tumor markers or inhibiting target for tumor treatment. In this study, embryonic rat livers were obtained to examine the expression of fascin and fibronectin during liver development. Then, the effect of fibronectin on fascin expression was investigated. At embryonic day (ED) 10.5, when the foregut endoderm began to form the liver bud and spread into the septum transversum, fibrous extracellular matrix was observed between the space where the liver bud and the septum transversum merged. At ED11.5, fibronectin was observed surrounding the cluster of fascin-positive hepatoblasts. At ED13.5, hematopoietic cells emerged and both fibronectin and fascin expression started to decline. Fascin and fibronectin appeared temporarily and disappeared by ED 14.5. Their expression was chronologically synchronized. Subsequently, the effect of fibronectin on fascin was examined by cultivation of hepatoblasts that were isolated from the ED13.5 rat liver. As a result, with fibronectin, fascin was positive in most hepatoblasts, although, without fibronectin, fascin expression was remarkably declined. Presently, there are few studies about the relationship between fascin and fibronectin. Our findings suggest that fibronectin could regulate fascin expression in rat hepatoblasts.