Intracellular immunization of human fetal cord blood stem/progenitor cells with a ribozyme against human immunodeficiency virus type 1.
Intracellular immunization of human fetal cord blood stem/progenitor cells with a ribozyme against human immunodeficiency virus type 1.
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使用针对人类免疫缺陷病毒 1 型的核酶对人胎儿脐带血干细胞/祖细胞进行细胞内免疫。
DOI：
10.1073/pnas.92.3.699
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发表时间：
1995
影响因子：
11.1
通讯作者：
Wong-Staal,F
中科院分区：
文献类型：
--
作者：
Yu,M;Leavitt,MC;Maruyama,M;Yamada,O;Young,D;Ho,AD;Wong-Staal,F
成功治疗人类免疫缺陷病毒感染可能最终需要靶向造血干细胞。本研究使用携带核酶基因的逆转录病毒载体转导人胎儿脐带血中的CD34+细胞。转导和核酶表达对细胞分化和/或增殖没有明显的不利影响。巨噬细胞样细胞在体外从干细胞/祖细胞中分化出来，表达核酶基因并抵抗嗜巨噬细胞型人类免疫缺陷病毒的感染。这些结果提示干细胞基因治疗人类免疫缺陷病毒感染患者的可行性。
Successful treatment of human immunodeficiency virus infection may ultimately require targeting of hematopoietic stem cells. Here we used retroviral vectors carrying the ribozyme gene to transduce CD34+ cells from human fetal cord blood. Transduction and ribozyme expression had no apparent adverse effect on cell differentiation and/or proliferation. The macrophage-like cells, differentiated from the stem/progenitor cells in vitro, expressed the ribozyme gene and resisted infection by a macrophage tropic human immunodeficiency virus type 1. These results suggest the feasibility of stem cell gene therapy for human immunodeficiency virus-infected patients.