Manipulation of culture conditions alters lipid content and fatty acid profiles of a wide variety of known and new oleaginous yeast species.
Manipulation of culture conditions alters lipid content and fatty acid profiles of a wide variety of known and new oleaginous yeast species.
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DOI：
10.1016/j.biortech.2013.06.047
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发表时间：
2013-09
影响因子：
11.4
通讯作者：
Boundy-Mills KL
中科院分区：
文献类型：
--
作者：
Sitepu IR;Sestric R;Ignatia L;Levin D;German JB;Gillies LA;Almada LA;Boundy-Mills KL
产油酵母在油脂化学生产中的研究已经超过80年。只有少数物种被深入研究。为了扩大可用于脂质研究的产油酵母的多样性，我们调查了具有产油性指标的酵母的广泛多样性，包括已知的产油分支和浮力。对17属50种69株产脂菌株进行了筛选。酵母菌属于子囊菌门、担子菌门和酵母样藻类无绿藻属。在不同孵育时间和氮利用率下测定细胞内总脂质和脂肪酸组成。发现了13种新的产油酵母菌，代表了多个子囊菌和担子菌分支。氮饥饿普遍增加细胞内脂质含量。脂肪酸谱随生长条件而变化，而不考虑分类归属。主要脂肪酸为油酸、棕榈酸、亚油酸和硬脂酸。酵母菌和培养条件，产生的脂肪酸适合生物柴油进行了鉴定。
Oleaginous yeasts have been studied for oleochemical production for over 80 years. Only a few species have been studied intensely. To expand the diversity of oleaginous yeasts available for lipid research, we surveyed a broad diversity of yeasts with indicators of oleaginicity including known oleaginous clades, and buoyancy. Sixty-nine strains representing 17 genera and 50 species were screened for lipid production. Yeasts belonged to Ascomycota families, Basidiomycota orders, and the yeast-like algal genus Prototheca. Total intracellular lipids and fatty acid composition were determined under different incubation times and nitrogen availability. Thirteen new oleaginous yeast species were discovered, representing multiple ascomycete and basidiomycete clades. Nitrogen starvation generally increased intracellular lipid content. The fatty acid profiles varied with the growth conditions regardless of taxonomic affiliation. The dominant fatty acids were oleic acid, palmitic acid, linoleic acid, and stearic acid. Yeasts and culture conditions that produced fatty acids appropriate for biodiesel were identified.