GENDER DIFFERENCES IN THE METABOLIC RESPONSE TO GRADED NUMBERS OF TRANSPLANTED ISLETS OF LANGERHANS
GENDER DIFFERENCES IN THE METABOLIC RESPONSE TO GRADED NUMBERS OF TRANSPLANTED ISLETS OF LANGERHANS
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DOI：
10.1210/en.135.6.2681
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发表时间：
1994-12-01
期刊：
影响因子：
4.8
通讯作者：
FINEGOOD, DT
中科院分区：
文献类型：
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作者：
BELL, RC;KHURANA, M;FINEGOOD, DT
性别差异治疗后与链脲佐菌素（STZ）已被报道，然而，在胰岛移植后，在STZ诱导的糖尿病的雄性和雌性大鼠胰岛移植的葡萄糖反应的差异还没有被检查。使用STZ（55 mg/kg BW）使雄性和雌性Wistar-Furth大鼠患糖尿病，然后给予门静脉内胰岛移植。对照组动物接受假注射和假移植手术;糖尿病动物接受STZ和假手术。在雄性动物中，胰岛移植物包含0（糖尿病），250，500，1000，1500，2000和3000个胰岛;在雌性大鼠中，移植物由0，500，700，750，1000或2500个胰岛组成。STZ治疗对雄性大鼠的影响比雌性大鼠更显着。在糖尿病阶段，与对照雄性动物相比，雄性大鼠的体重显著降低;在雌性动物中未观察到这一情况。尽管所有STZ治疗的动物都是高血糖的，但在此阶段，雄性糖尿病大鼠的血糖水平显著高于雌性大鼠（29.8 ± 2.1 us. 24.6+/-0.6mM）。胰岛移植后，雄性大鼠体重增加与移植的胰岛数呈正相关（r（2）= 0.59; P < 0.01），而雌性大鼠体重增加与移植的胰岛数无相关性（r（2）= 0.09; P > 0.8）。在雄性和雌性大鼠中，接受1000个或更多胰岛的动物在移植后3周通常血糖正常（雄性，10.8 +/- 2.2 mM;雌性，7.1 +/- 0.2 mM）。接受500个胰岛的雄性和雌性动物中，约60%的动物实现了血糖水平的降低。雌性动物的平均血糖水平为17.2 +/- 2.3 mM，雄性动物为22.6 +/- 1.0 mM。然而，与接受500个胰岛的雄性动物（30只中的2只）相比，500个胰岛的雌性动物（16只中的6只）中血糖水平达到9.5 mM或更低的比例显著更大。多元回归分析表明，性别和胰岛数量交互影响胰岛移植后血糖正常化。性别差异似乎会影响体重和血糖对胰岛移植的反应。这一发现可能具有特殊的相关性时，边际数量的功能性胰岛可用。
Gender differences after treatment with streptozotocin (STZ) have been previously reported; however, differences in the glucose response to islet transplantation in STZ-induced diabetes in male and female rats after islet transplantation have not been examined. Male and female Wistar-Furth rats were made diabetic using STZ (55 mg/kg BW) and then given an intraportal islet transplant. Control animals received sham injections and sham transplant surgery; diabetic animals received STZ and sham surgery. In male animals, islet grafts contained 0 (diabetic), 250, 500, 1000, 1500, 2000, and 3000 islets; in female rats, grafts were made up of 0, 500 700, 750, 1000, or 2500 islets. STZ treatment had more dramatic effects on male than female rats. During the diabetic phase, body weights of male rats were significantly reduced compared to those of control male animals; this was not observed among females. Although all STZ-treated animals were hyperglycemic, plasma glucose levels in male diabetic rats were significantly higher than those in females during this phase (29.8 +/- 2.1 us. 24.6 +/- 0.6 mM). After islet transplantation, body weight gain was positively associated with the number of islets transplanted in male rats (r(2) = 0.59; P < 0.01), but not in females (r(2) = 0.09; P > 0.8). In both male and female rats, animals that received 1000 islets or more were generally normoglycemic by 3 weeks posttransplant (males, 10.8 +/- 2.2 mM; females, 7.1 +/- 0.2 mM). Approximately 60% of male and female animals that received 500 islets achieved a reduction in plasma glucose levels. Mean plasma glucose levels were 17.2 +/- 2.3 in the females and 22.6 +/- 1.0 mM in males. However, a significantly larger proportion of female 500-islet animals (6 of 16) achieved a plasma glucose level of 9.5 mM or less compared with males receiving 500 islets (2 of 30). Multivariate regression analysis suggests that sex and islet number interact to affect glycemic normalization after islet transplantation. Gender differences appear to influence body weight and plasma glucose responses to islet transplantation. This finding may have particular relevance when a marginal number of functional islets are available.