Photosynthetic Activity of Chloroplasts Isolated From Bermudagrass (Cynodon dactylon L.), a Species With a High Photosynthetic Capacity.
Photosynthetic Activity of Chloroplasts Isolated From Bermudagrass (Cynodon dactylon L.), a Species With a High Photosynthetic Capacity.
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DOI：
10.1104/pp.44.5.649
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发表时间：
1969-05
期刊：
影响因子：
7.4
通讯作者：
T. M. Chen;R. Brown;C. C. Black-C.
中科院分区：
文献类型：
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作者：
T. M. Chen;R. Brown;C. C. Black-C.
从狗牙根(Cynodon dactylon L.)并测定叶片的光合磷酸化和电子传递活性。这些叶绿体在与吩嗪甲硫酸盐的循环电子流和非循环电子流的同时，积极地合成三磷酸腺苷，同时还原了烟酰胺、腺苷二核苷酸磷酸、细胞色素c和铁氰化物等Hill氧化剂。磷和二磷酸腺苷的表观Km值分别为5×10~(-5)M和2.5×10~(-5)M。研究了光强对光磷酸化的影响，计算了环磷酸化与非环磷酸化的摩尔比。结果表明，狗牙根叶片的高光合作用能力可能是由这些叶绿体研究表明的光磷酸化能力所支持的，而叶绿体研究中异常缺乏关于在高光强下产生足够的CO(2)同化还原剂的数据。
Chloroplasts have been isolated from bermudagrass (Cynodon dactylon L.) leaves and assayed for photophosphorylation and electron transport activity. These chloroplasts actively synthesize adenosine triphosphate during cyclic electron flow with phenazine methosulfate and noncyclic electron flow concurrent with the reduction of such Hill oxidants as nicotinamide adenosine dinucleotide phosphate, cytochrome c, and ferricyanide. Apparent Km values for the cofactors of photophosphorylation have been determined to be 5 x 10(-5) M for phosphate and 2.5 x 10(-5) M for adenosine diphosphate. The influence of light intensity on photophosphorylation has been studied and the molar ratio of cyclic to noncyclic phosphorylation calculated. It is concluded that the high photosynthetic capacity of bermudagrass leaves probably could be supported by the photophosphorylation capacities indicated in these chloroplast studies and the anomalous lack of data in chlorolast studies on the production of sufficient reductant for CO(2) assimilation at high light intensities has been noted.