Free solution mobility of DNA molecules containing variable numbers of cationic phosphoramidate internucleoside linkages.
Free solution mobility of DNA molecules containing variable numbers of cationic phosphoramidate internucleoside linkages.
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含有不同数量的阳离子氨基磷酸酯核苷间键合的 DNA 分子的自由溶液迁移率。
DOI：
10.1016/s0021-9673(00)00415-5
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Righetti,PG
中科院分区：
文献类型：
--
作者：
Stellwagen,NC;Magnusdottir,S;Dagle,JM;Gelfi,C;Righetti,PG
用毛细管电泳法测定了含有0、3、6或9个阳离子磷酰胺核苷键的118个碱基对DNA片段的自由溶液电泳率。正如预期的那样，由于DNA分子上的负电荷减少，电泳迁移率随着阳离子磷酰胺键的增加而降低。对于含有三个和六个阳离子磷酰胺键的DNA分子来说，迁移率的下降大致是线性的，但当添加了九个阳离子磷酰胺键后，迁移率的下降开始趋于平稳。迁移率也有所不同，这取决于修饰的磷酰胺键是位于DNA分子的5‘端还是3’端。
The free solution electrophoretic mobility of an 118-base pair DNA fragment containing zero, three, six or nine cationic phosphoramidate internucleoside linkages has been measured by capillary electrophoresis. The electrophoretic mobility decreases with the increasing number of cationic phosphoramidate linkages, as expected because of the reduced negative charge on the DNA molecules. The decrease in mobility is approximately linear for DNA molecules containing three and six cationic phosphoramidate linkages, but begins to level off when nine cationic phosphoramidate linkages have been added. The mobility also varies somewhat depending on whether the modified phosphoramidate linkages are located at the 5′- or 3′-end of the DNA molecule.