Co-Amended Synergistic Interactions between Arbuscular Mycorrhizal Fungi and the Organic Substrate-Induced Cucumber Yield and Fruit Quality Associated with the Regulation of the AM-Fungal Community Structure under Anthropogenic Cultivated Soil
Co-Amended Synergistic Interactions between Arbuscular Mycorrhizal Fungi and the Organic Substrate-Induced Cucumber Yield and Fruit Quality Associated with the Regulation of the AM-Fungal Community Structure under Anthropogenic Cultivated Soil
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DOI：
10.3390/ijms20071539
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发表时间：
2019-03-27
影响因子：
5.6
通讯作者：
Iqbal, Muhammad
中科院分区：
文献类型：
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作者：
Ali, Ahmad;Ghani, Muhammad Imran;Iqbal, Muhammad
温室蔬菜栽培（PGVC）下的黄瓜一年两熟种植系统由于长期人为活动和人为调控而受到严重影响，导致作物生产力下降和土壤生物区系紊乱。在这项研究中，土著丛枝菌根菌株（AM：Glomus versiforme L.）和有机基质（GS：在温室（2016-2018年）中，在特征为重植退化土壤的栽培人为土下，评估了大蒜茎（大蒜茎）施用的植物微生物相互作用和作物生产力反馈。结果表明，在有机基质（GS）中多次添加AM菌剂能促进黄瓜的生长和生理。AM与C-改良有机基质共生的有益性状优先表现为增加根系定殖、菌丝密度增殖、AM产孢、根系活力和抑制镰刀菌发病。AM后处理进一步增强了协同效应，共接种效应提高了果实营养吸收量、黄瓜产量和果实品质。Illumina MiSeq分析的18 S rRNA基因扩增子显示，占主导地位的AM属，特别是丰富的球囊霉分类群可能是重要的生态驱动因素与植物生产力和果实品质特性。这些结果表明，AM-有机基质协会可能是一个务实的选择，作为一个经济和有效的生物资源，并作为一个新的可持续的植物微生物介质，以提高区域生态系统服务和植物生产力的人为PGVC的该地区。
Monotonous cucumber double-cropping systems under plastic greenhouse vegetable cultivation (PGVC) previously intensified by long-term anthropogenic activities and manipulative treatments leads to a crop productivity reduction and soil biota disturbances. In this study, the role of the indigenous arbuscular mycorrhizal strain (AM: Glomus versiforme L.) and organic substrate (GS: Garlic stalk) application were assessed for plant microbe interaction and crop productivity feedback in a greenhouse (2016-2018) under a cultivated Anthrosol characterized as a replanted degraded soil. We found that repetitively adding AM inocula with organic substrates (GS) improved the cucumber growth and physiology. The useful trait of AM symbiosis with C-amended organic substrates preferentially manifested as increased root colonization, hyphal density proliferation, AM sporulation, root activity, and suppressed Fusarium incidence. The post AM development further prevailed the synergistic interaction, and the co-inoculation effect resulted in an increase in fruit nutrition uptake, seasonal cucumber yield and fruit quality attributes. Illumina MiSeq analysis of the 18S rRNA gene amplicons revealed that the dominant AM genera that are particularly enriched with the Glomus taxon may be important ecological drivers associated with plant productivity and fruit quality characteristics. These results suggest that the AM-organic substrate association might be a pragmatic option for use as an economic and efficient biological resource and as a newly-sustainable plant microbe mediator to enhance the regional ecosystem services and plant productivity of the anthropogenic PGVC of this region.