Inhibition of Glucose-Induced Insulin Secretion in Trypsin-Treated Islets of Langerhans
Inhibition of Glucose-Induced Insulin Secretion in Trypsin-Treated Islets of Langerhans
复制标题
胰蛋白酶处理的胰岛中葡萄糖诱导的胰岛素分泌的抑制
DOI：
10.1055/s-0028-1093936
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发表时间：
1973
影响因子：
2.2
通讯作者：
A. Wacker
中科院分区：
文献类型：
--
作者：
U. Krause *;H. Puchinger;A. Wacker
本文报道了一种从大鼠胰岛中分离单细胞的方法。在这些细胞中，胰岛素分泌可以用磺酰脲类药物刺激，但不能用葡萄糖。在通过额外的胰蛋白酶处理制备的细胞中，两种刺激剂都无效。用胰蛋白酶预处理完整胰岛可抑制葡萄糖诱导的胰岛素分泌，而甲苯磺丁脲诱导的胰岛素释放不受影响。用不含Ca 2 +、Mg 2的缓冲液预处理胰岛也会抑制随后在二价阳离子存在下孵育期间葡萄糖诱导的胰岛素释放。这些结果表明，胰岛的形态完整性不是胰岛素释放的先决条件。葡萄糖诱导的胰岛素释放对胰岛的胰蛋白酶处理的敏感性进一步支持葡萄糖受体模型。
A method is described for the preparation of single cells from isolated rat islets by treatment of the islets with a ea 2 +, Mg 2 +-free buffer and mild shearing forres. In these cells insulin secretion can be stimulated with sulfonylureas, but not with glucose. In cells prepared by an additional trypsin treatment both stimulants are ineffective. Pretreat ment of intact islets with trypsin inhibits glucose-induced insulin secretion, whereas tolbutamide-induced insulin relca~e is unaffected. Pretreatment of islets with Ca 2 +, Mg 2 -free buffer also inhibits glucose induced insulin release during the subsequent incubation in the presence of divalent cations. These results indicate, that the morphological in tegrity of the islets is no prerequisite to insulin release. The sensit ivity of glucose-induced insulin release to trypsin treatment of the islets further supports the glucoreceptor model.