Towards passive bioremediation of dye-bearing effluents using hydrous ferric oxide wastes: Mechanisms, products and microbiology.
Towards passive bioremediation of dye-bearing effluents using hydrous ferric oxide wastes: Mechanisms, products and microbiology.
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DOI：
10.1016/j.jenvman.2022.115332
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发表时间：
2022-05
影响因子：
8.7
通讯作者：
Pallavee Srivastava;Safa Al-Obaidi;G. Webster;A. Weightman;D. Sapsford
中科院分区：
文献类型：
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作者：
Pallavee Srivastava;Safa Al-Obaidi;G. Webster;A. Weightman;D. Sapsford
提出了一种新颖的、受循环经济启发的“被动”（无动力和无试剂）处理含染料废水的方法。该处理利用了含染料废水与含水合氧化铁（HFO）污泥的生物地球化学相互作用。这项工作首次展示了饮用水和矿井水处理中富含氢氟烯烃的废物污泥的再利用。在简单的流通式反应器中，直接利用废水作为介质/基质，对甲基橙子（MO）和混合染料纺织废水进行脱色和生物降解。进行了三个阶段的探索性概念验证工作。用MO溶液和用甘油修正的MO（阶段I）、合成纺织品流出物配方中的MO（阶段II）和含有染料混合物的真实的混合纺织品流出物（阶段III）挑战含有HFO污泥的柱。在最初的滞后期后，在所有情况下观察到染料的广泛脱色，其速率与纯菌株和工程生物反应器工艺相当，有证据表明生物降解超出了单偶氮发色团和矿化的简单裂解。在这两种情况下，初始污泥样品的微生物学表现出各种各样的铁氧化和还原细菌。但实验后污泥的微生物学由变形菌属（Proteobacteria）为主演变为厚壁菌属（Firmicutes）为主。在处理后的MWTS和WTWS中观察到微生物群落结构的明显变化，其中确定了能够进行铁和硫酸盐还原和/或芳香胺降解的属。柱的平均脱氮率范围为27.8至194 g/m3/天，高于工程连续厌氧-好氧生物反应器。快速厌氧脱色、生物降解和染料矿化（以及氮转化）的假定机制包括各种直接和间接的酶促和代谢反应，以及不断再生的还原剂（如Fe（II）、HFO结合的Fe（II）、FeS和HS−）的还原攻击。铁还原剂降解芳环的能力也被认为在有机碳的进一步生物降解和完全矿化中很重要。该研究表明，丰富和无处不在的富含HFO的废物污泥，可以使用没有修正案，作为一个简单的流通生物修复系统的脱色和部分生物降解的染料在纺织废水的基板。
A novel, circular economy-inspired approach for the “passive” (non-powered and reagent-free) treatment of dye-bearing effluent is presented. The treatment utilises the biogeochemical interaction of dye-bearing wastewater with hydrous ferric oxide (HFO) bearing sludges. The work presented demonstrates for the first time the reuse of HFO-rich waste sludges from potable water and mine water treatment. The waste was used directly without modification or reagent addition, as media/substrate in simple flow-through reactors for the decolourisation and biodegradation of methyl orange (MO) and mixed dyes textile effluent. Three phases of exploratory proof of concept work were undertaken. Columns containing HFO sludges were challenged with solution of MO, and MO amended with glycerol (Phase I), MO in a synthetic textile effluent recipe (Phase II), and real mixed textile effluent containing a mixture of dyes (Phase III). After an initial lag period extensive decolourisation of dye was observed in all cases at rates comparable with pure strains and engineered bioreactor processes, with evidence of biodegradation beyond simple cleavage of the mono azo chromophore and mineralisation. The microbiology of the initial sludge samples in both cases exhibited a diverse range of iron oxidising and reducing bacteria. However, post experiment the microbiology of sludge evolved from being dominated byProteobacteriato being dominated byFirmicutes. Distinct changes in the microbial community structure were observed in post-treatment MWTS and WTWS where genera capable of iron and sulphate reduction and/or aromatic amine degradation were identified. Average nitrogen removal rates for the columns ranged from 27.8 to 194 g/m3/day which is higher than engineered sequential anaerobic-aerobic bioreactor. Postulated mechanisms for the fast anaerobic decolourisation, biodegradation, and mineralisation of the dyes (as well nitrogen transformations) include various direct and indirect enzymatic and metabolic reactions, as well as reductive attack by continuously regenerated reductants such as Fe(II), HFO bound Fe(II), FeS, and HS−. The ability of iron reducers to degrade aromatic rings is also considered important in the further biodegradation and complete mineralisation of organic carbon. The study reveals that abundant and ubiquitous HFO-rich waste sludges, can be used without amendment, as a substrate in simple flow-through bioremediation system for the decolourisation and partial biodegradation of dyes in textile effluent.