Evaluation of acute phase protein indexes in dogs with leishmaniasis at diagnosis, during and after short-term treatment
Evaluation of acute phase protein indexes in dogs with leishmaniasis at diagnosis, during and after short-term treatment
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利什曼病犬诊断时、短期治疗期间和治疗后急性期蛋白指标的评价
DOI：
10.17221/5595-vetmed
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发表时间：
2018
影响因子：
0.7
通讯作者：
J. Cerón
中科院分区：
文献类型：
--
作者：
S. Martínez;J. Cerón
本研究开发并评价了一种基于急性时相蛋白组合的急性时相指数，该指数允许监测犬利什曼病对治疗的反应。6只自然感染婴儿利什曼原虫的犬接受锑酸葡甲胺（Glucantime β-葡聚糖，Merial，里昂，法国）100 mg/kg/天皮下给药，同时给予别嘌呤醇（Zyloric β-葡聚糖，Glaxo Wellcome，马德里，西班牙）30 mg/kg/天口服给药20天，然后以相同剂量单独给予别嘌呤醇1个月。在治疗开始前和治疗开始后的不同日期采集急性时相蛋白的血样，并计算两组指标：（1）合并一种阳性和一种阴性急性时相蛋白的指标和（2）合并两种阳性和一种阴性急性时相蛋白的指标。与临床健康犬（n = 8）相比，利什曼病动物的所有计算指数均显著较高，并且在给药期间，在所有受试犬中均观察到下降。C-反应蛋白（CRP）和铜蓝蛋白（CP）与其他蛋白质相结合的指标显示出更大的百分比下降，具有统计学意义。其中，CRP*CP/Alb指数被选为最佳值，因为与其他指标以及单独的单个蛋白质相比，它显示出更大和更快的下降。这些结果将支持使用选定的急性期指数，特别是CRP*CP/Alb指数，怀疑利什曼病犬，并监测其对治疗的反应。
An acute phase index based on a combination of acute phase proteins which permitted monitor- ing the response to therapy of canine leishmaniasis was developed and evaluated in this study. Six dogs naturally infected by Leishmania infantum were treated with meglumine antimoniate (Glucantime , Merial, Lyon, France) 100 mg/kg/day sc, given concurrently for 20 days with allopurinol (Zyloric , Glaxo Wellcome, Madrid, Spain) 30 mg/kg/day po and then, allopurinol alone for one month at the same dosage. Blood samples for acute phase proteins were obtained on different days before and after the beginning of treatment and two groups of indexes were calculated: (1) Indexes that combined one positive and one negative acute phase protein and (2) Indexes that combined two positive and one negative acute phase proteins. All calculated indexes were significantly higher in animals with leishmaniasis compared with clinically healthy dogs (n = 8) and a decrease was observed in all dogs tested during the treatment. Indexes that combined C-reactive protein (CRP) and ceruloplasmin (CP) with other proteins showed greater percentages of decrease that were statistically significant. Among these, the index CRP*CP/Alb was selected as the optimum since it showed a larger and faster decrease compared with the others as well as with individual proteins alone. These results would support the use of selected acute phase indexes, especially the CRP*CP/Alb index, to suspect about a leishmaniotic dogs and to monitor their response to treatment.