Differential display and gene arrays to examine auditory plasticity
Differential display and gene arrays to examine auditory plasticity
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DOI：
10.1016/s0378-5955(00)00139-8
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发表时间：
2000-09-01
期刊：
影响因子：
2.8
通讯作者：
Altschuler, RA
中科院分区：
文献类型：
--
作者：
Lomax, MI;Huang, L;Altschuler, RA
差异基因表达构成组织和器官发育、分化、再生和可塑性的基础。我们描述了两种识别差异表达基因的方法。差异显示：一种基于 PCR 的方法，比较两种或多种条件下基因子集的表达。基因阵列或 DNA 微阵列包含来自已知基因和点在固体支持物（尼龙膜或载玻片）上的新基因的 cDNA。阵列与从两种不同实验条件中分离的RNA杂交可以同时分析大量基因，从数百到数千到整个基因组。使用差异显示检查噪音损伤后雏鸡基底乳头的差异基因表达？我们鉴定了编码 E3 泛素连接酶家族 (UBE3B) 新成员的 UBE3B 基因。 UBE3B 在雏鸡基底乳头的病变部位发出噪音后立即高度表达，但在未受损的末端不表达。 UBE3B 与来自秀丽隐杆线虫的泛素连接酶基因最相似，表明该基因在整个进化过程中一直是保守的。我们还描述了利用尼龙膜上市售的低密度基因阵列来分析耳蜗和大脑中基因表达的初步实验，并讨论了该技术和 DNA 微阵列技术在听觉系统中的潜在应用。 (C) 2000 Elsevier Science B.V. 保留所有权利。
Differential gene expression forms the basis for development, differentiation, regeneration, and plasticity of tissues and organs. We describe two methods to identify differentially expressed genes. Differential display: a PCR-based approach, compares the expression of subsets of genes under two or more conditions. Gene arrays, or DNA microarrays, contain cDNAs from both known genes and novel genes spotted on a solid support (nylon membranes or glass slides). Hybridization of the arrays with RNA isolated from two different experimental conditions allows the simultaneous analysis of large numbers of genes, from hundreds to thousands to whole genomes. Using differential display to examine differential gene expression after noise trauma in the chick basilar papilla? we identified the UBE3B gene that encodes a new member of the E3 ubiquitin ligase family (UBE3B). UBE3B is highly expressed immediately after noise in the lesion, but not in the undamaged ends, of the chick basilar papilla. UBE3B is most similar to a ubiquitin ligase gene from Caenorhabditis elegans, suggesting that this gene has been conserved throughout evolution. We also describe preliminary experiments to profile gene expression in the cochlea and brain with commercially available low density gene arrays on nylon membranes and discuss potential applications of this and DNA microarray technology to the auditory system. (C) 2000 Elsevier Science B.V. All rights reserved.