Efficacy of Pseudomonas fluorescens strains and some modern fungicides for control of Rhizoctonia solani AG-4 in bean and cucumber

Efficacy of Pseudomonas fluorescens strains and some modern fungicides for control of Rhizoctonia solani AG-4 in bean and cucumber
复制标题

荧光假单胞菌菌株和一些现代杀菌剂对大豆和黄瓜中立枯丝核菌 AG-4 的防治效果

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发表时间:
2002
期刊:
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影响因子:
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通讯作者:
H. Buchenauer
H. Buchenauer
中科院分区:
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文献类型:
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作者:
H. Kataria;B. Wilmsmeier;H. Buchenauer

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60株荧光假单胞菌在KB-琼脂上进行体外试验,并在腐殖土:砂混合物中用立枯丝核菌AG-4的分离物侵染,25株对大豆幼苗的立枯病和根腐病提供了中等程度的保护,但在黄瓜中只有相当中等或较差的病害控制。在11种杀菌剂中,嘧菌酯、咯菌腈和戊唑醇在25 mg/kg i. kg和1 g a. i. 50-100 mg/kg戊唑醇和1 g/kg甲基立枯磷对菜豆的防效均在90%以上。较低比率的嘧菌酯、咯菌腈、嘧菌隆和戊唑醇作为种子处理与土壤浸液的抗性诱导化学品(5-硝基水杨酸、邻乙酰水杨酸、2-氨基异丁酸、地衣素)或与荧光假单胞菌菌株W36的组合在菜豆和黄瓜中的病害控制方面没有任何显著改善;这些组合中的大多数表现出拮抗相互作用。讨论了荧光假单胞菌、杀菌剂及其与诱导抗性的化学物质的组合处理对病害控制的可能机制和意义,沿着对豆类和黄瓜病害控制的不同选择的相对功效的关键评估。
Of 60 strains of Pseudomonas fluorescens tested in vitro on KB-agar and as soil drench in Humosoil : sand mix infested with an isolate of Rhizoctonia solani AG-4, 25 provided moderately good protection of bean seedlings against damping-off and root rot, but only fairly moderate or a poor disease control in cucumber. Of 11 fungicides tested as seed treatment, azoxystrobin, fludioxonil and tebuconazole at 25 mg a. i./kg and iprodione at 1 g a. i./kg gave > 90 % disease control in cucumber; tebuconazole (50-100 mg/kg) and tolclofos-methyl ( 1 g/kg) gave ≥ 90 % disease control in bean. Lower rates of azoxystrobin, fludioxonil, pencycuron and tebuconazole applied as seed treatment in combinations with soil drenches of resistance-inducing chemicals (5-nitrosalicylic acid, o-acetylsalicylic acid, 2-aminoisobutyric acid, lichenin) or with P. fluorescens strain W36 did not result in any significant improvement in disease control in bean and cucumber; most of these combinations showed antagonistic interaction. Probable mechanisms and implications of disease control by P. fluorescens strains, fungicides and their combined treatments with resistance-inducing chemicals are discussed, along with critical assessment of the relative efficacy of the different options for disease control in bean and cucumber.