Altered transcription of glutamatergic and glycinergic receptors in spinal cord dorsal horn following spinal cord transection is minimally affected by passive exercise of the hindlimbs
Altered transcription of glutamatergic and glycinergic receptors in spinal cord dorsal horn following spinal cord transection is minimally affected by passive exercise of the hindlimbs
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DOI：
10.1111/ejn.13823
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发表时间：
2018-02-01
影响因子：
3.4
通讯作者：
Gardiner, Phillip F.
中科院分区：
文献类型：
--
作者：
Chopek, Jeremy W.;MacDonell, Christopher W.;Gardiner, Phillip F.
基因表达改变脊髓横断（STx）后，在运动和感觉系统。运动已被证明会影响STx后两个系统中的基因表达。在不完全性脊髓损伤（SCI）或挫伤性SCI后，在脊髓背根神经节和伤害性椎板中也显示出基因表达的改变。然而，STx和运动对脊髓I-III层基因表达的影响尚未得到充分研究。因此，本研究的目的是确定是否在板层I-III的基因表达改变后STx，并确定是否叠加被动运动的后肢会影响基因表达后STx板层I-III。激光捕获显微切割选择性收获板层I-III的腰椎脊髓切片，并使用定量RT-PCR检测23个选定的基因在对照组，STx和STx加运动大鼠的样品中的相对表达。我们表明，后STx，代谢型谷氨酸受体1，5和8的基因表达上调，而离子型谷氨酸受体（谷氨酸2）和甘氨酸能亚基GLRA 1的表达下调。每日运动减弱了I-III层Glur 2基因表达的下调。我们的研究结果表明，在STx模型中，基因表达在板层I-III中改变，并且尽管被动运动影响运动和感觉系统中的基因表达，但它对STx后板层I-III中的基因表达的影响最小。
Gene expression is altered following a spinal transection (STx) in both motor and sensory systems. Exercise has been shown to influence gene expression in both systems post-STx. Gene expression alterations have also been shown in the dorsal root ganglia and nociceptive laminae of the spinal cord following either an incomplete spinal cord injury (SCI) or a contusive SCI. However, the effect of STx and exercise on gene expression in spinal cord laminae I-III has not fully been examined. Therefore, the purpose of this study was to determine whether gene expression in laminae I-III is altered following STx and determine whether superimposed passive exercise of the hindlimbs would influence gene expression post-STx in laminae I-III. Laser capture microdissection was used to selectively harvest laminae I-III of lumbar spinal cord sections, and quantitative RT-PCR was used to examine relative expression of 23 selected genes in samples collected from control, STx and STx plus exercise rats. We demonstrate that post-STx, gene expression for metabotropic glutamate receptors 1, 5 and 8 were up-regulated, whereas ionotropic glutamatergic receptor (Glur2) and glycinergic subunit GLRA1 expression was down-regulated. Daily exercise attenuated the down-regulation of Glur2 gene expression in laminae I-III. Our results demonstrate that in a STx model, gene expression is altered in laminae I-III and that although passive exercise influences gene expression in both the motor and sensory systems, it had a minimal effect on gene expression in laminae I-III post-STx.