Murine SPAM1 is secreted by the estrous uterus and oviduct in a form that can bind to sperm during capacitation: acquisition enhances hyaluronic acid-binding ability and cumulus dispersal efficiency
Murine SPAM1 is secreted by the estrous uterus and oviduct in a form that can bind to sperm during capacitation: acquisition enhances hyaluronic acid-binding ability and cumulus dispersal efficiency
复制标题
DOI：
10.1530/rep-07-0340
复制
发表时间：
2008-03-01
期刊：
影响因子：
3.8
通讯作者：
Martin-DeLeon, Patricia A.
中科院分区：
文献类型：
--
作者：
Griffiths, Genevieve S.;Miller, Kimberly A.;Martin-DeLeon, Patricia A.
最近表明，精子在体外摄取附睾精子粘附分子 1 (SPAM1) 是精子成熟的标志，因为获得这种表面透明质酸酶可提高卵丘分散效率。在这里，我们证明了这种糖基磷脂酰肌醇连接的精子抗原（之前显示在女性生殖道的动情期间表达）以 67 kDa 的形式分泌在子宫和输卵管液（分别为 ULF 和 OF）中，可以与精子结合。我们证明，通过免疫细胞化学和流式细胞术检测，它可以通过 Spam1 缺失、Spam1 缺陷突变体和野生型 (WT) 小鼠的尾部精子在 37°C ULF 或 OF 中孵育期间体外获得。 ULF 孵育后，SPAM1 结合主要定位于 Spam1 无效精子的顶体和鞭毛中段，其模式与 WT 精子相同。 ULF 孵育后，WT 精子表现出显着的 (P
Sperm uptake of epididymal sperm adhesion molecule 1 (SPAM1) in vitro has recently been shown to be a marker of sperm maturation, since acquisition of this surface hyaluronidase increases cumulus dispersal efficiency. Here, we demonstrate that this glycosyl phosphatidylinositol-linked sperm antigen, previously shown to be expressed during estrous in the female reproductive tract, is secreted in the uterine and oviductal fluids (ULF and OF respectively) in a 67 kDa form, which can bind to sperm. We show that it can be acquired by caudal sperm from Spam1 null, Spam1-deficient mutant, and wild-type (WT) mice in vitro during incubation in ULF or OF at 37 degrees C, as detected by immunocytochemistry and flow cytometry. SPAM1 binding after ULF incubation was localized predominantly to the acrosome and the mid-piece of the flagella of Spam1 null sperm in a pattern identical to that of WT sperm. After ULF incubation, WT sperm demonstrated a significantly (P