INSITU HYBRIDIZATION STUDIES OF STROMELYSIN AND COLLAGENASE MESSENGER-RNA EXPRESSION IN RHEUMATOID SYNOVIUM
INSITU HYBRIDIZATION STUDIES OF STROMELYSIN AND COLLAGENASE MESSENGER-RNA EXPRESSION IN RHEUMATOID SYNOVIUM
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DOI：
10.1002/art.1780340903
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发表时间：
1991-09-01
影响因子：
--
通讯作者：
GLIMCHER, LH
中科院分区：
文献类型：
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作者：
GRAVALLESE, EM;DARLING, JM;GLIMCHER, LH
类风湿关节炎（RA）中的破坏性关节变化被认为部分是由中性蛋白酶胶原酶和溶基质素介导的。胶原酶信使 RNA (mRNA) 先前已定位于滑膜内层。在这项研究中，通过原位杂交检查了 8 名 RA 患者和 2 名骨关节炎患者的滑膜组织中蛋白酶的产生情况。溶基质素 mRNA 主要定位于滑膜衬里层细胞。在连续切片中，胶原酶 mRNA 被证明定位于与产生溶基质素 mRNA 的区域相同的组织区域，并且颗粒计数揭示了溶基质素 mRNA 的产生与胶原酶 mRNA 的产生之间的直接相关性。所有 RA 患者均产生可检测量的胶原酶和溶基质素 mRNA。两名骨关节炎患者中的一名产生这些金属蛋白酶，但其水平低于 RA 患者的水平。这些数据支持滑膜衬里细胞作为RA中产生胶原酶和溶基质素的主要滑膜细胞的身份，并为RA体内胶原酶和溶基质素的协调生产提供新证据。
Destructive joint changes in rheumatoid arthritis (RA) are thought to be mediated in part by the neutral proteinases collagenase and stromelysin. Collagenase messenger RNA (mRNA) has been previously localized to the synovial lining layer. In this study, synovial tissue from 8 patients with RA and 2 patients with osteoarthritis was examined for proteinase production by in situ hybridization. Stromelysin mRNA localized predominantly to the synovial lining layer cells. In serial sections, collagenase mRNA was shown to be localized to the same tissue areas as those producing stromelysin mRNA, and grain counts revealed a direct correlation between production of stromelysin mRNA and production of collagenase mRNA. All patients with RA were producing collagenase and stromelysin mRNA in detectable amounts. One of 2 osteoarthritis patients was producing these metalloproteinases, but in levels below those found in the RA patients. These data support the identity of the synovial lining cells as the major synovial cells producing collagenase and stromelysin in RA and provide new evidence for the coordinate production of collagenase and stromelysin in RA in vivo.