Hartman interferometer: versatile integrated optic sensor for label-free, real-time quantification of nucleic acids, proteins, and pathogens.
Hartman interferometer: versatile integrated optic sensor for label-free, real-time quantification of nucleic acids, proteins, and pathogens.
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DOI：
10.1093/clinchem/43.9.1757
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发表时间：
1997-09
影响因子：
9.3
通讯作者：
B. H. Schneider;J. G. Edwards;N. Hartman
中科院分区：
文献类型：
--
作者：
B. H. Schneider;J. G. Edwards;N. Hartman
Hartman干涉仪是一种专有的集成光学传感器，为广泛的生物医学诊断提供了基础，包括基于抗体和基于基因探针的检测。与其他倏逝波光学传感器一样，干涉仪测量波导表面生物分子结合引起的折射率变化。一般来说，倏逝波传感器令人兴奋的前景在于，它们有可能被用作无标签、实时的传感器，可以以真正的混合和读取方式运行，并提供快速、定量的结果。然而，其开发面临的主要问题之一是为多分析物测试创建一个简单、低成本的配置。哈特曼干涉仪通过依赖线偏振光和平面波导格式解决了这一挑战，从而避免了与圆偏振和通道波导相关的问题。我们报告初步实验，证明这种传感器配置的适用性，以检测广泛的蛋白质，核酸和病原体分析。
The Hartman interferometer, a proprietary integrated optic sensor, provides a basis for a broad range of biomedical diagnostics, including antibody-based and gene probe-based assays. As with other evanescent-wave optical sensors, the interferometer measures the refractive index change resulting from biomolecular binding on a waveguide surface. The exciting promise of evanescent-wave sensors lies, in general, in their potential to be used as label-free, real-time transducers that can operate in a true mix-and-read fashion and provide fast, quantitative results. One of the major issues facing their development, however, is creating a simple, low-cost configuration for multianalyte testing. The Hartman interferometer addresses this challenge by relying on linearly polarized light and a planar waveguide format, thereby avoiding the problems associated with circular polarization and channel waveguides. We report preliminary experiments that demonstrate the applicability of this sensor configuration to detection of a wide range of protein, nucleic acid, and pathogen analytes.