Serum MicroRNAs as Diagnostic Biomarkers for Macrosomia
Serum MicroRNAs as Diagnostic Biomarkers for Macrosomia
复制标题
DOI：
10.1177/1933719114561557
复制
发表时间：
2015-06-01
影响因子：
2.9
通讯作者：
Dong, Jing
中科院分区：
文献类型：
--
作者：
Jiang, Hua;Wen, Yang;Dong, Jing
背景：巨大儿定义为婴儿出生体重超过4000 g。尽管microRNAs（miRNAs）与多种疾病的发病机制有关，但血清miRNAs与巨大儿的关系却鲜有报道。方法：我们采用Taqman低密度芯片结合定量逆转录聚合酶链反应（RT-PCR）技术，对分娩前1周收集的血清样本进行检测，筛选与巨大儿相关的miRNAs。分析结果显示，在巨大儿（Ct > 3倍）的血清样品中，1种miRNA显著上调，10种miRNA显著下调。与对照组相比，巨大儿中miR-21的表达水平在妊娠晚期显著降低。接受者工作特征（ROC）曲线分析显示，miR-21的ROC曲线下面积为67.7%（灵敏度为66.7%，特异性为70.0%）。结论：miR-21在巨大儿和对照组孕妇血清中的表达存在差异，miR-21可作为预测巨大儿的候选生物标志物。
Background: Macrosomia is defined as an infant's birth weight of more than 4000 g. Although microRNAs (miRNAs) have been implicated in the pathogenesis of various diseases, the associations between serum miRNAs and macrosomia have been rarely reported.Methodology: We used the Taqman Low Density Array followed by quantitative reverse transcriptase polymerase chain reaction assays to screen for miRNAs associated with macrosomia using serum samples collected 1 week before delivery.Results: Profiling results showed that 1 miRNA was significantly upregulated and 10 miRNAs were significantly downregulated in serum samples of macrosomia (Ct > 3-fold). The expression levels of miR-21 were significantly decreased in macrosomia as compared to the controls in the third trimester. Receiver operating characteristic (ROC) curve analyses showed that the area under the ROC curve for miR-21 was 67.7% (sensitivity = 66.7% and specificity = 70.0%).Conclusions: miR-21 in maternal serum is differentially expressed between macrosomia and controls, and miR-21 could be used as a candidate biomarker to predict macrosomia.