In Vitro Bioavailability, Cellular Antioxidant Activity, and Cytotoxicity of β-Carotene-Loaded Emulsions Stabilized by Catechin-Egg White Protein Conjugates
In Vitro Bioavailability, Cellular Antioxidant Activity, and Cytotoxicity of β-Carotene-Loaded Emulsions Stabilized by Catechin-Egg White Protein Conjugates
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DOI：
10.1021/acs.jafc.7b05909
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发表时间：
2018-02-21
影响因子：
6.1
通讯作者：
Yang, Yanjun
中科院分区：
文献类型：
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作者：
Gu, Luping;Pan, Che;Yang, Yanjun
以前，它表明，儿茶素卵白色蛋白（CT-EWP）共轭物是有效的抗氧化剂乳化剂，可以形成和稳定的乳液，也抑制包封类胡萝卜素的降解。本研究的目的是评价偶联对β-胡萝卜素乳剂体外生物利用度、细胞抗氧化活性和细胞毒性的影响。包被有EWP或CT-EWP结合物的脂滴在通过模拟胃肠道时表现出非常相似的行为。包封在由CT-EWP缀合物稳定的乳液中的β-胡萝卜素表现出更高的总体体外生物利用度，这归因于类胡萝卜素对化学转化的更大稳定性。CT-EWP结合物稳定的乳剂在基于细胞的测定（二氯荧光素二乙酸酯）中也表现出更大的抑制氧化的能力。细胞毒性分析表明，由CT-EWP缀合物稳定的β-胡萝卜素乳剂在高浓度下使用时仅表现出轻微的细胞毒性。这些结果表明，CT-EWP缀合物可用于配制具有增强的抗氧化活性和生物利用度的化学不稳定的疏水生物活性物质的基于乳液的递送系统。
Previously, it was shown that catechin egg white protein (CT-EWP) conjugates were effective antioxidant emulsifiers that could form and stabilize emulsions, and also inhibit the degradation of encapsulated carotenoids. The objective of the current study was to evaluate the impact of conjugation on the in vitro bioavailability, cellular antioxidant activity, and cytotoxicity of beta-carotene-loaded emulsions. Lipid droplets coated with EWP or with CT-EWP conjugates exhibited quite similar behavior when they were passed through a simulated gastrointestinal tract. The beta-carotene encapsulated in emulsions stabilized by CT-EWP conjugates exhibited a higher overall in vitro bioavailability, which was attributed to a greater stability of the carotenoids to chemical transformation. The emulions stabilized by CT-EWP conjugates also exhibited greater ability in inhibiting oxidation in a cell-based assay (dichlorofluorescein diacetate). Cytotoxicity analysis suggested that beta-carotene emulsions stabilized by CT-EWP conjugates only exhibited a slight cytotoxicity when used at high concentrations. These results suggest that CT-EWP conjugates can be used to formulate emulsion-based delivery systems for chemically labile hydrophobic bioactives with enhanced antioxidant activity and bioavailability.