Rapid detection of Epstein-Barr virus DNA by loop-mediated isothermal amplification method
Rapid detection of Epstein-Barr virus DNA by loop-mediated isothermal amplification method
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DOI：
10.1016/j.jcv.2006.07.011
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发表时间：
2006-10-01
影响因子：
8.8
通讯作者：
Kimura, Hiroshi
中科院分区：
文献类型：
--
作者：
Iwata, Seiko;Shibata, Yukiko;Kimura, Hiroshi
背景：环介导等温扩增（LAMP）方法是一种新的特异性DNA序列扩增技术。目的：建立EB病毒（EBV）DNA的LAMP扩增方法，并探讨其在临床标本中检测EBV DNA的可靠性。研究设计：对108例疑似原发性EBV感染的患者血清进行EBV LAMP检测，并与荧光定量PCR检测结果进行比较。以血清学检查为标准诊断方法。结果：LAMP诊断原发性EBV感染的敏感性为86.4%，特异性为100%。实时荧光定量PCR检测的敏感性为84.1%，特异性为98.4%。纵向分析显示，随着EBV载量的降低，LAMP法对血清中EBV DNA的检出率也随着时间的延长而降低。发病后40天血清中未检测到EBV DNA。结论：这些结果表明，LAMP方法的灵敏度和特异性与实时PCR的灵敏度和特异性相当，并且通过该方法检测血清中的EBV DNA对于诊断原发性EBV感染是潜在有用的。(C)2006 Elsevier B.V.保留所有权利。
Background: The loop-mediated isothermal amplification (LAMP) method is a novel technique for the amplification of specific DNA sequences. Objectives: To establish the LAMP method for amplifying Epstein-Barr virus (EBV) DNA and to examine its reliability for the detection of EBV DNA in clinical specimens. Study design: Sera from 108 patients, who were initially suspected of primary EBV infection, were tested by the EBV LAMP method, and the results were compared with those of the real-time PCR assay. Serological examination was regarded as the standard diagnostic method. Results: To diagnose primary EBV infection, the sensitivity of LAMP was 86.4% and the specificity was 100%. The sensitivity of the real-time PCR assay was 84.1 % and the specificity was 98.4%. Longitudinal analysis showed that the detection rate of EBV DNA in serum by the LAMP method decreased with time in accordance with the decrease of the EBV load. EBV DNA could not be detected in serum 40 days after onset of symptoms. Conclusions: These results indicate that the sensitivity and specificity of the LAMP method are comparable to those of real-time PCR and that detecting EBV DNA in serum by this method is potentially useful for diagnosing primary EBV infection. (C) 2006 Elsevier B.V. All rights reserved.