Differential expression of regulator of G-protein signalling transcripts and in vivo migration of CD4+ naive and regulatory T cells
Differential expression of regulator of G-protein signalling transcripts and in vivo migration of CD4+ naive and regulatory T cells
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DOI：
10.1111/j.1365-2567.2005.02146.x
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发表时间：
2005-06-01
期刊：
影响因子：
6.4
通讯作者：
Ceredig, R
中科院分区：
文献类型：
--
作者：
Agenès, F;Bosco, N;Ceredig, R
T淋巴细胞对病原体的免疫应答在引流的次级淋巴器官中启动，然后活化的细胞迁移到感染部位。因此，控制幼稚和调节性CD 4（+）T细胞迁移是至关重要的;然而，在生理和病理条件下对其了解甚少。我们发现，CD 4（+）亚群显示G蛋白信号（RGS）基因表达谱的特征调节。调节性T细胞比初始细胞表达更高水平的RGS 1、RGS 9和RGS 16。这些基因在细胞活化后上调，并且它们的表达水平与体内细胞迁移相关。使用联体共生，我们表明，调节性T淋巴细胞迁移比幼稚T细胞和迁移幼稚T细胞表达甚至更低的RGS水平比他们的静态同行。我们的研究结果表明，幼稚和调节性T细胞的迁移能力和RGS 1，RGS 9和RGS 16的水平之间呈负相关。总之，这些结果表明RGS分子在趋化因子诱导的淋巴细胞迁移中的作用，并证明了调节性T细胞在表型和迁移能力方面的特殊性，为其功能提供了新的见解。
The immune response of T lymphocytes to pathogens is initiated in draining secondary lymphoid organs, and activated cells then migrate to the site of infection. Thus, control of naive and regulatory CD4(+) T-cell migration is crucial; however, it is poorly understood in physiological and pathological conditions. We found that CD4(+) subpopulations displayed characteristic regulator of G-protein signalling (RGS) gene expression profiles. Regulatory T cells express higher levels of RGS1, RGS9 and RGS16 than naive cells. These genes are up-regulated upon cell activation and their level of expression correlates with in vivo cell migration. Using parabiosis, we showed that regulatory T lymphocytes migrate less than naive T cells and that migrant naive T cells express even lower RGS levels than their static counterparts. Our results show an inverse correlation between the capacity to migrate and the levels of RGS1, RGS9 and RGS16 for both naive and regulatory T cells. Taken together, these results suggest a role for RGS molecules in chemokine-induced lymphocyte migration and demonstrate the peculiarity of regulatory T cells in terms of phenotype and migration ability, providing new insights into their function.