The use of protocatechuate dioxygenase for maintaining anaerobic conditions in biochemical experiments
The use of protocatechuate dioxygenase for maintaining anaerobic conditions in biochemical experiments
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DOI：
10.1006/abio.2000.4802
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发表时间：
2000-11-15
影响因子：
2.9
通讯作者：
Ballou, DP
中科院分区：
文献类型：
--
作者：
Patil, PV;Ballou, DP
氧化还原活性系统的研究通常需要维持厌氧条件。葡萄糖氧化酶系统通常用于维持厌氧条件，但它有一些缺点，例如产生 H2O2 和稳定性限制。来自洋葱伯克霍尔德菌的原儿茶酸双加氧酶和底物原儿茶酸构成了另一种有效的氧洗涤系统，可用于各种生化实验。我们已经证明其适合在 pH 6-9 的溶液中、温度 4 至 35 摄氏度下维持严格的厌氧环境，时间长达 15 个月。该酶系统在这些条件下表现出稳定性，并且可有效维持 FAD 滴定中的厌氧条件。它还适用于擦洗各种类型的仪器，例如用于厌氧实验的停流仪器。 (C) 2000 年学术出版社。
The study of redox-active systems often requires the maintenance of anaerobic conditions. The glucose oxidase system has often been used to maintain anaerobic conditions, but it has some drawbacks, such as the production of H2O2 and limitations on stability. Protocatechuate dioxygenase from Burkholderia cepacia and the substrate, protocatechuate, constitute an alternate effective oxygen-scrubbing system that can be used in a wide variety of biochemical experiments. We have shown its suitability for maintaining rigorous anaerobic environments in solutions of pH 6-9, at temperatures from 4 to 35 degreesC, and for periods of time up to 15 months. The enzyme system was shown to be stable under these conditions and effective for maintaining anaerobic conditions in titrations of FAD. It is also suitable for scrubbing various types of apparatus such as stopped-flow instruments for anaerobic experiments. (C) 2000 Academic Press.