MICROCOLUMN SEPARATIONS AND THE ANALYSIS OF SINGLE CELLS
MICROCOLUMN SEPARATIONS AND THE ANALYSIS OF SINGLE CELLS
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DOI：
10.1126/science.2675314
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发表时间：
1989-10-06
期刊：
影响因子：
56.9
通讯作者：
JORGENSON, JW
中科院分区：
文献类型：
--
作者：
KENNEDY, RT;OATES, MD;JORGENSON, JW
毛细管区带电泳和开管液相色谱是被称为微柱分离的分析仪器的新兴领域的两个例子。这些方法的高分辨率和小样品要求使其适用于单细胞的定量、多组分化学分析。适当的仪器分析纳升和亚纳升样品进行了讨论。从单个神经元的分析数据，包括氨基酸和神经递质的含量。
Capillary zone electrophoresis and open tubular liquid chromatography are two examples of an emerging area of analytical instrumentation known as microcolumn separations. The high resolution and small sample requirements of these methods make them suitable for the quantitative, multicomponent chemical analysis of single cells. Appropriate instrumentation for the analysis of nanoliter and subnanoliter samples is discussed. Data from the analysis of individual neurons are presented, including amino acid and neurotransmitter content.