ISOLATION AND CHARACTERIZATION OF PARACOCCUS-DENITRIFICANS MUTANTS WITH DEFECTS IN THE METABOLISM OF ONE-CARBON COMPOUNDS
ISOLATION AND CHARACTERIZATION OF PARACOCCUS-DENITRIFICANS MUTANTS WITH DEFECTS IN THE METABOLISM OF ONE-CARBON COMPOUNDS
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DOI：
10.1128/jb.164.3.1064-1070.1985
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发表时间：
1985-01-01
影响因子：
3.2
通讯作者：
STOUTHAMER, AH
中科院分区：
文献类型：
--
作者：
HARMS, N;DEVRIES, GE;STOUTHAMER, AH
用诱变剂n -甲基-n " -硝基-n -亚硝基胍处理反硝化副球菌获得了缺乏单碳化合物代谢的突变体。通过新开发的平板筛选试验选择突变体，无需富集程序。获得的突变体通过其生长反应、细胞色素组成、酶活性和抗血清对甲醇脱氢酶的免疫原性反应进行了表征。根据这些标准可以区分出5个突变类。一类突变体参与甲醇脱氢酶的表达。这一类的三个突变体在结构基因上有缺陷。发现了甲醇脱氢酶和氢化酶表达缺陷的双突变体。II类突变体在涉及甲醇脱氢酶和甲胺脱氢酶的调控基因中存在缺陷。III类突变体缺乏甲醛代谢。一个缺陷可能存在于第二非nad1连接甲醛脱氢酶的表达，这被认为是参与C1代谢。IV类突变体缺乏细胞色素c。V类突变体在合成甲酸脱氢酶功能所必需的钼辅助因子方面存在缺陷。
Mutants deficient in the metabolism of one-carbon compounds have been obtained by treating Paracoccus denitrificans with the mutagen N-methyl-N''-nitro-N-nitrosoguanidine. Mutants were selected without enrichment procedures by newly developed plate screening tests. The obtained mutants were characterized by their growth responses, cytochrome composition, enzyme activities, and immunogenic reaction with antisera against methanol dehydrogenase. By these criteria five mutant classes could be distinguished. Class I mutants are involved in the expression of methanol dehydrogenase. Three mutants of this class have a defect in the structural gene. A double mutants was found with defects in the expression of both methanol dehydrogenase and hydrogenase. Class II mutants have a defect in a regulatory gene involved in the regulation of both methanol dehydrogenase and methylamine dehydrogenase. Class III mutants are deficient in formaldehyde metabolism. A defect may exist in the expression of a second non-NAD-linked formaldehyde dehydrogenase which was postulated to be involved in C1 metabolism. Class IV mutants are deficient in cytochrome c. Mutants of class V have a defect in synthesis of the molybdenum cofactor essential for the function of formate dehydrogenase.