Twenty-seven nonoverlapping zinc finger cDNAs from human T cells map to nine different chromosomes with apparent clustering.
Twenty-seven nonoverlapping zinc finger cDNAs from human T cells map to nine different chromosomes with apparent clustering.
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发表时间：
1991-04
影响因子：
9.8
通讯作者：
K. Huebner;T. Druck;C. Croce;H. Thiesen
中科院分区：
文献类型：
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作者：
K. Huebner;T. Druck;C. Croce;H. Thiesen
通过筛选具有锌指共有序列的Molt 4和Jurkat cDNA文库来分离编码锌指结构的cDNA克隆。对候选克隆进行部分测序以验证锌指编码区的存在;根据序列和基因组杂交模式选择不重叠的cDNA克隆。编码锌指结构的克隆，其用术语“Kox”和1至32的数字表示，并且其显然是独特的（即，彼此不同且与其他实验室分离的那些不同）被选择用于在人类基因组中作图。从啮齿动物-人的体细胞杂交保留人类染色体的定义互补的DNA进行了分析的存在下，每个Kox基因。特定人类染色体区域和特定Kox基因的存在之间的相关性建立了染色体位置。多个Kox基因定位于7 q（Kox 18和25以及由Kox 8 cDNA和Kox 27 cDNA检测到的基因座），8 q24 5'到myc基因座（Kox 9和32），10 cen-q24（Kox 2，15，19，21，30和31），12 q13-qter（Kox 1和20），17 p13（Kox 11和26）和19 q（Kox 5，6，10，22，24和28）。单个Kox基因座定位于IG lambda和BCR-1之间的7 p22（Kox 3）、18 q12（Kox 17）、19 p（Kox 13）、22 q11（Kox 8 cDNA和Kox 27 cDNA均检测到的基因座）和Xp（Kox 14）。几个Kox基因座映射到其他锌指结构编码基因座已经定位的区域，表明可能的锌指基因簇。此外，位于8 q24、17 p13和22 q11的Kox基因--也许还有其他Kox基因--位于复发性染色体易位断点附近。其他的，如7 p和7 q上的，可能靠近T细胞中特异性活性的区域。
cDNA clones encoding zinc finger structures were isolated by screening Molt4 and Jurkat cDNA libraries with zinc finger consensus sequences. Candidate clones were partially sequenced to verify the presence of zinc finger-encoding regions; nonoverlapping cDNA clones were chosen on the basis of sequences and genomic hybridization pattern. Zinc finger structure-encoding clones, which were designated by the term "Kox" and a number from 1 to 32 and which were apparently unique (i.e., distinct from each other and distinct from those isolated by other laboratories), were chosen for mapping in the human genome. DNAs from rodent-human somatic cell hybrids retaining defined complements of human chromosomes were analyzed for the presence of each of the Kox genes. Correlation between the presence of specific human chromosome regions and specific Kox genes established the chromosomal locations. Multiple Kox loci were mapped to 7q (Kox 18 and 25 and a locus detected by both Kox 8 cDNA and Kox 27 cDNA), 8q24 5' to the myc locus (Kox 9 and 32), 10cen----q24 (Kox 2, 15, 19, 21, 30, and 31), 12q13-qter (Kox 1 and 20), 17p13 (Kox 11 and 26), and 19q (Kox 5, 6, 10, 22, 24, and 28). Single Kox loci were mapped to 7p22 (Kox 3), 18q12 (Kox 17), 19p (Kox 13), 22q11 between IG lambda and BCR-1 (locus detected by both Kox 8 cDNA and Kox 27 cDNA), and Xp (Kox 14). Several of the Kox loci map to regions in which other zinc finger structure-encoding loci have already been localized, indicating possible zinc finger gene clusters. In addition, Kox genes at 8q24, 17p13, and 22q11--and perhaps other Kox genes--are located near recurrent chromosomal translocation breakpoints. Others, such as those on 7p and 7q, may be near regions specifically active in T cells.